Application of Mie theory to determine the structure of spheroidal scatterers in biological materials.

Published

Journal Article

We present here the results of a numerical study on light scattering from nonspherical particles with relevance to detecting precancerous states in epithelial tissues. In previous studies of epithelial cell nuclei, the experimental light scattering data have been analyzed by comparison with Mie theory. However, given the spheroidal shape of many cell nuclei, the validity of this assumption demands a thorough investigation. We investigate this assumption by using the T-matrix method to model light scattered from spheroids with parameters relevant to epithelial cell nuclei. In our previous studies, we have developed a data analysis procedure that extracts the oscillatory component of the angular-scattering distribution for an ensemble of epithelial cell nuclei for comparison with Mie theory. We demonstrate that application of our analysis procedure to the predictions of the T-matrix method for spheroids, oriented such that their axis of symmetry is aligned with the incident light propagation direction, generally yields the spheroid dimension that is transverse to the incident light propagation direction with subwavelength accuracy.

Full Text

Duke Authors

Cited Authors

  • Keener, JD; Chalut, KJ; Pyhtila, JW; Wax, A

Published Date

  • May 2007

Published In

Volume / Issue

  • 32 / 10

Start / End Page

  • 1326 - 1328

PubMed ID

  • 17440576

Pubmed Central ID

  • 17440576

Electronic International Standard Serial Number (EISSN)

  • 1539-4794

International Standard Serial Number (ISSN)

  • 0146-9592

Digital Object Identifier (DOI)

  • 10.1364/ol.32.001326

Language

  • eng