Application of the T-matrix method to determine the structure of spheroidal cell nuclei with angle-resolved light scattering.


Journal Article

We demonstrate an inverse light-scattering analysis procedure based on using the T-matrix method as a light-scattering model. We measure light scattered by in vitro cell monolayers using angle-resolved low-coherence interferometry (a/LCI) and compare the data to predictions of the T-matrix theory. The comparison yields measurements of the equal volume diameter and aspect ratio of the spheroid cell nuclei with accuracy comparable to quantitative image analysis of fixed and stained samples. These improvements represent a significant upgrade for the a/LCI technique, expanding both the range of tissue in which it is applicable and potentially increasing its value as a diagnostic tool.

Full Text

Duke Authors

Cited Authors

  • Giacomelli, MG; Chalut, KJ; Ostrander, JH; Wax, A

Published Date

  • November 2008

Published In

Volume / Issue

  • 33 / 21

Start / End Page

  • 2452 - 2454

PubMed ID

  • 18978884

Pubmed Central ID

  • 18978884

Electronic International Standard Serial Number (EISSN)

  • 1539-4794

International Standard Serial Number (ISSN)

  • 0146-9592

Digital Object Identifier (DOI)

  • 10.1364/ol.33.002452


  • eng