Quantitative phase microscopy with asynchronous digital holography.

Journal Article (Journal Article)

We demonstrate a new method of measuring quantitative phase in imaging of biological materials. This method, asynchronous digital holography, employs knowledge of a moving fringe created by acousto-optic modulators to execute phase-shifting interferometry using two near-simultaneous interferograms. The method can be used to obtain quantitative phase images of dynamic biological samples on millisecond time scales. We present results on a standard sample, and on live cell samples.

Full Text

Duke Authors

Cited Authors

  • Chalut, KJ; Brown, WJ; Wax, A

Published Date

  • March 2007

Published In

Volume / Issue

  • 15 / 6

Start / End Page

  • 3047 - 3052

PubMed ID

  • 19532543

Pubmed Central ID

  • PMC2832294

Electronic International Standard Serial Number (EISSN)

  • 1094-4087

International Standard Serial Number (ISSN)

  • 1094-4087

Digital Object Identifier (DOI)

  • 10.1364/oe.15.003047


  • eng