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Identification of novel mutations and sequence variants in the SOX2 and CHX10 genes in patients with anophthalmia/microphthalmia.

Publication ,  Journal Article
Zhou, J; Kherani, F; Bardakjian, TM; Katowitz, J; Hughes, N; Schimmenti, LA; Schneider, A; Young, TL
Published in: Mol Vis
March 24, 2008

PURPOSE: Mutations in the SOX2 and CHX10 genes have been reported in patients with anophthalmia and/or microphthalmia. In this study, we evaluated 34 anophthalmic/microphthalmic patient DNA samples (two sets of siblings included) for mutations and sequence variants in SOX2 and CHX10. METHODS: Conformational sensitive gel electrophoresis (CSGE) was used for the initial SOX2 and CHX10 screening of 34 affected individuals (two sets of siblings), five unaffected family members, and 80 healthy controls. Patient samples containing heteroduplexes were selected for sequence analysis. Base pair changes in SOX2 and CHX10 were confirmed by sequencing bidirectionally in patient samples. RESULTS: Two novel heterozygous mutations and two sequence variants (one known) in SOX2 were identified in this cohort. Mutation c.310 G>T (p. Glu104X), found in one patient, was in the region encoding the high mobility group (HMG) DNA-binding domain and resulted in a change from glutamic acid to a stop codon. The second mutation, noted in two affected siblings, was a single nucleotide deletion c.549delC (p. Pro184ArgfsX19) in the region encoding the activation domain, resulting in a frameshift and premature termination of the coding sequence. The shortened protein products may result in the loss of function. In addition, a novel nucleotide substitution c.*557G>A was identified in the 3'-untranslated region in one patient. The relationship between the nucleotide change and the protein function is indeterminate. A known single nucleotide polymorphism (c. *469 C>A, SNP rs11915160) was also detected in 2 of the 34 patients. Screening of CHX10 identified two synonymous sequence variants, c.471 C>T (p.Ser157Ser, rs35435463) and c.579 G>A (p. Gln193Gln, novel SNP), and one non-synonymous sequence variant, c.871 G>A (p. Asp291Asn, novel SNP). The non-synonymous polymorphism was also present in healthy controls, suggesting non-causality. CONCLUSIONS: These results support the role of SOX2 in ocular development. Loss of SOX2 function results in severe eye malformation. CHX10 was not implicated with microphthalmia/anophthalmia in our patient cohort.

Duke Scholars

Published In

Mol Vis

EISSN

1090-0535

Publication Date

March 24, 2008

Volume

14

Start / End Page

583 / 592

Location

United States

Related Subject Headings

  • Transcription Factors
  • SOXB1 Transcription Factors
  • Polymorphism, Single Nucleotide
  • Ophthalmology & Optometry
  • Mutation
  • Microphthalmos
  • Male
  • Humans
  • Homeodomain Proteins
  • Heterozygote
 

Citation

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Zhou, J., Kherani, F., Bardakjian, T. M., Katowitz, J., Hughes, N., Schimmenti, L. A., … Young, T. L. (2008). Identification of novel mutations and sequence variants in the SOX2 and CHX10 genes in patients with anophthalmia/microphthalmia. Mol Vis, 14, 583–592.
Zhou, Jie, Femida Kherani, Tanya M. Bardakjian, James Katowitz, Nkecha Hughes, Lisa A. Schimmenti, Adele Schneider, and Terri L. Young. “Identification of novel mutations and sequence variants in the SOX2 and CHX10 genes in patients with anophthalmia/microphthalmia.Mol Vis 14 (March 24, 2008): 583–92.
Zhou J, Kherani F, Bardakjian TM, Katowitz J, Hughes N, Schimmenti LA, et al. Identification of novel mutations and sequence variants in the SOX2 and CHX10 genes in patients with anophthalmia/microphthalmia. Mol Vis. 2008 Mar 24;14:583–92.
Zhou J, Kherani F, Bardakjian TM, Katowitz J, Hughes N, Schimmenti LA, Schneider A, Young TL. Identification of novel mutations and sequence variants in the SOX2 and CHX10 genes in patients with anophthalmia/microphthalmia. Mol Vis. 2008 Mar 24;14:583–592.

Published In

Mol Vis

EISSN

1090-0535

Publication Date

March 24, 2008

Volume

14

Start / End Page

583 / 592

Location

United States

Related Subject Headings

  • Transcription Factors
  • SOXB1 Transcription Factors
  • Polymorphism, Single Nucleotide
  • Ophthalmology & Optometry
  • Mutation
  • Microphthalmos
  • Male
  • Humans
  • Homeodomain Proteins
  • Heterozygote