Characterization of cultured thymus tissue used for transplantation with emphasis on promiscuous expression of thyroid tissue-specific genes.

Published

Journal Article

Autoimmune thyroid disease occurs in some complete DiGeorge anomaly patients after thymus transplantation. This study was designed to assess the effect of culture of thymus tissue on the expression of genes involved in the development of autoimmunity. The expression of autoimmune regulator (AIRE), thyroglobulin (TG), thyroid peroxidase (TPO), and cytokeratin RNAs was examined in thymocytes and thymus tissue on the day of thymus harvest and after 14 and 21 days of culture. Immunohistochemistry was used to evaluate the cytokeratin expression in the thymus tissue. AIRE, TG, TPO, and cytokeratin mRNAs were found in harvest-day, 14-day and 21-day cultured tissues. Levels of AIRE, TG, and cytokeratin mRNAs were mostly higher after culture compared to expression on the harvest day, likely secondary to thymocyte depletion.

Full Text

Duke Authors

Cited Authors

  • Li, B; Li, J; Hsieh, C-S; Hale, LP; Li, Y-J; Devlin, BH; Markert, ML

Published Date

  • January 2009

Published In

Volume / Issue

  • 44 / 1-3

Start / End Page

  • 71 - 83

PubMed ID

  • 19066738

Pubmed Central ID

  • 19066738

Electronic International Standard Serial Number (EISSN)

  • 1559-0755

International Standard Serial Number (ISSN)

  • 0257-277X

Digital Object Identifier (DOI)

  • 10.1007/s12026-008-8083-4

Language

  • eng