Skip to main content
Journal cover image

Modified vaccinia virus Ankara can activate NF-kappaB transcription factors through a double-stranded RNA-activated protein kinase (PKR)-dependent pathway during the early phase of virus replication.

Publication ,  Journal Article
Lynch, HE; Ray, CA; Oie, KL; Pollara, JJ; Petty, ITD; Sadler, AJ; Williams, BRG; Pickup, DJ
Published in: Virology
September 1, 2009

Modified vaccinia virus Ankara (MVA), which is a promising replication-defective vaccine vector, is unusual among the orthopoxviruses in activating NF-kappaB transcription factors in cells of several types. In human embryonic kidney (HEK 293T) cells, the MVA-induced depletion of IkappaBalpha required to activate NF-kappaB is inhibited by UV-inactivation of the virus, and begins before viral DNA replication. In HEK 293T, CHO, or RK13 cells, expression of the cowpox virus CP77 early gene, or the vaccinia virus K1L early gene suppresses MVA-induced IkappaBalpha depletion. In mouse embryonic fibroblasts (MEFs), MVA induction of IkappaBalpha depletion is dependent on the expression of mouse or human double-stranded RNA-activated protein kinase (PKR). These results demonstrate that events during the early phase of MVA replication can induce PKR-mediated processes contributing both to the activation of NF-kappaB signaling, and to processes that may restrict viral replication. This property may contribute to the efficacy of this vaccine virus.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Virology

DOI

EISSN

1096-0341

Publication Date

September 1, 2009

Volume

391

Issue

2

Start / End Page

177 / 186

Location

United States

Related Subject Headings

  • eIF-2 Kinase
  • Virus Replication
  • Virology
  • Vaccinia virus
  • NF-kappa B
  • NF-KappaB Inhibitor alpha
  • Mice
  • I-kappa B Proteins
  • Humans
  • Cricetinae
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Lynch, H. E., Ray, C. A., Oie, K. L., Pollara, J. J., Petty, I. T. D., Sadler, A. J., … Pickup, D. J. (2009). Modified vaccinia virus Ankara can activate NF-kappaB transcription factors through a double-stranded RNA-activated protein kinase (PKR)-dependent pathway during the early phase of virus replication. Virology, 391(2), 177–186. https://doi.org/10.1016/j.virol.2009.06.012
Lynch, Heather E., Caroline A. Ray, Katrina L. Oie, Justin J. Pollara, Ian T. D. Petty, Anthony J. Sadler, Bryan R. G. Williams, and David J. Pickup. “Modified vaccinia virus Ankara can activate NF-kappaB transcription factors through a double-stranded RNA-activated protein kinase (PKR)-dependent pathway during the early phase of virus replication.Virology 391, no. 2 (September 1, 2009): 177–86. https://doi.org/10.1016/j.virol.2009.06.012.
Journal cover image

Published In

Virology

DOI

EISSN

1096-0341

Publication Date

September 1, 2009

Volume

391

Issue

2

Start / End Page

177 / 186

Location

United States

Related Subject Headings

  • eIF-2 Kinase
  • Virus Replication
  • Virology
  • Vaccinia virus
  • NF-kappa B
  • NF-KappaB Inhibitor alpha
  • Mice
  • I-kappa B Proteins
  • Humans
  • Cricetinae