Major expansion of CD8+ T cells with a predominant V beta usage during the primary immune response to HIV.

Published

Journal Article

A SIGNIFICANT proportion (up to 70%) of individuals experience an acute clinical syndrome of varying severity associated with primary infection with the human immunodeficiency virus (HIV). We report here studies on six individuals who showed an acute HIV syndrome which generally resolved within four weeks, concomitant with a dramatic downregulation of viraemia. To characterize the T-cell-mediated primary immune response to HIV, we used combined semiquantitative polymerase chain reaction assay and cytofluorometry to analyse the T-cell antigen receptor repertoire in sequential peripheral blood mononuclear cells from the patients. We found major oligoclonal expansions in a restricted set of variable-domain beta-chain (V beta) families. Cells expressing the expanded V beta s predominantly expressed the CD8 T-cell differentiation antigen and mediated HIV-specific cytotoxicity. Major oligoclonal expansions of these CD8+ T lymphocytes may represent an important component of the primary immune response to viral infections and may help to clarify both the immunopathogenic and the protective mechanisms of HIV infection.

Full Text

Duke Authors

Cited Authors

  • Pantaleo, G; Demarest, JF; Soudeyns, H; Graziosi, C; Denis, F; Adelsberger, JW; Borrow, P; Saag, MS; Shaw, GM; Sekaly, RP

Published Date

  • August 1994

Published In

Volume / Issue

  • 370 / 6489

Start / End Page

  • 463 - 467

PubMed ID

  • 8047166

Pubmed Central ID

  • 8047166

Electronic International Standard Serial Number (EISSN)

  • 1476-4687

International Standard Serial Number (ISSN)

  • 0028-0836

Digital Object Identifier (DOI)

  • 10.1038/370463a0

Language

  • eng