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Precise arrangement of factor-binding sites is required for murine CD4 promoter function.

Publication ,  Journal Article
Sarafova, S; Siu, G
Published in: Nucleic Acids Res
July 15, 2000

The control of CD4 expression is linked to the signaling events that mediate T-cell development and is directly dependent on the CD4 promoter. The CD4 promoter does not contain functionally redundant sites: all four factor-binding sites must be intact to achieve wild-type activity. Here we demonstrate that the precise position of three factor-binding sites relative to each other is essential for promoter activity, indicating that they function together as an inseparable cassette for assembly of the transcription initiation complex. Small changes in either phasing or distance between any two sites in this cassette leads to complete abrogation of promoter function. In addition, we demonstrate that one of the factors that bind the promoter cassette is not present in CD8 SP T(C) cells. Thus, this factor is a candidate for mediating the relative subclass specificity of CD4 promoter function in activated CD4 SP T(H) cells.

Duke Scholars

Published In

Nucleic Acids Res

DOI

EISSN

1362-4962

Publication Date

July 15, 2000

Volume

28

Issue

14

Start / End Page

2664 / 2671

Location

England

Related Subject Headings

  • Recombinant Fusion Proteins
  • Protein Binding
  • Promoter Regions, Genetic
  • Plasmids
  • Mutagenesis, Site-Directed
  • Mice
  • Luciferases
  • Developmental Biology
  • DNA-Binding Proteins
  • DNA
 

Citation

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Sarafova, S., & Siu, G. (2000). Precise arrangement of factor-binding sites is required for murine CD4 promoter function. Nucleic Acids Res, 28(14), 2664–2671. https://doi.org/10.1093/nar/28.14.2664
Sarafova, S., and G. Siu. “Precise arrangement of factor-binding sites is required for murine CD4 promoter function.Nucleic Acids Res 28, no. 14 (July 15, 2000): 2664–71. https://doi.org/10.1093/nar/28.14.2664.
Sarafova S, Siu G. Precise arrangement of factor-binding sites is required for murine CD4 promoter function. Nucleic Acids Res. 2000 Jul 15;28(14):2664–71.
Sarafova, S., and G. Siu. “Precise arrangement of factor-binding sites is required for murine CD4 promoter function.Nucleic Acids Res, vol. 28, no. 14, July 2000, pp. 2664–71. Pubmed, doi:10.1093/nar/28.14.2664.
Sarafova S, Siu G. Precise arrangement of factor-binding sites is required for murine CD4 promoter function. Nucleic Acids Res. 2000 Jul 15;28(14):2664–2671.
Journal cover image

Published In

Nucleic Acids Res

DOI

EISSN

1362-4962

Publication Date

July 15, 2000

Volume

28

Issue

14

Start / End Page

2664 / 2671

Location

England

Related Subject Headings

  • Recombinant Fusion Proteins
  • Protein Binding
  • Promoter Regions, Genetic
  • Plasmids
  • Mutagenesis, Site-Directed
  • Mice
  • Luciferases
  • Developmental Biology
  • DNA-Binding Proteins
  • DNA