A single step purification for autolytic zinc proteinases.

Published

Journal Article

We describe a novel single-step method for the purification of stromelysin-1 catalytic domain (SCD) via immobilized metal affinity chromatography under denaturing conditions that inhibit proteolytic activity followed by on-column refolding and spontaneous autolysis of the fusion peptide to yield pure, active stromelysin-1 catalytic domain. The methodology provides a general approach for the rapid purification of large quantities of zinc proteinases.

Full Text

Published version (via Digital Object Identifier)
Pubmed Central version

Duke Authors

Toone, Eric John

Cited Authors

Wilfong, EM; Locklear, U; Toone, EJ

Published Date

January 2010

Published In

Bioorganic & Medicinal Chemistry Letters

Volume / Issue

20 / 1

Start / End Page

280 - 282

PubMed ID

19942433

Pubmed Central ID

19942433

Electronic International Standard Serial Number (EISSN)

1464-3405

International Standard Serial Number (ISSN)

0960-894X

Digital Object Identifier (DOI)

10.1016/j.bmcl.2009.10.114

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