A single step purification for autolytic zinc proteinases.
Journal Article (Journal Article)
We describe a novel single-step method for the purification of stromelysin-1 catalytic domain (SCD) via immobilized metal affinity chromatography under denaturing conditions that inhibit proteolytic activity followed by on-column refolding and spontaneous autolysis of the fusion peptide to yield pure, active stromelysin-1 catalytic domain. The methodology provides a general approach for the rapid purification of large quantities of zinc proteinases.
Full Text
Duke Authors
Cited Authors
- Wilfong, EM; Locklear, U; Toone, EJ
Published Date
- January 2010
Published In
Volume / Issue
- 20 / 1
Start / End Page
- 280 - 282
PubMed ID
- 19942433
Pubmed Central ID
- PMC3688063
Electronic International Standard Serial Number (EISSN)
- 1464-3405
International Standard Serial Number (ISSN)
- 0960-894X
Digital Object Identifier (DOI)
- 10.1016/j.bmcl.2009.10.114
Language
- eng