A single step purification for autolytic zinc proteinases.

Journal Article (Journal Article)

We describe a novel single-step method for the purification of stromelysin-1 catalytic domain (SCD) via immobilized metal affinity chromatography under denaturing conditions that inhibit proteolytic activity followed by on-column refolding and spontaneous autolysis of the fusion peptide to yield pure, active stromelysin-1 catalytic domain. The methodology provides a general approach for the rapid purification of large quantities of zinc proteinases.

Full Text

Duke Authors

Cited Authors

  • Wilfong, EM; Locklear, U; Toone, EJ

Published Date

  • January 2010

Published In

Volume / Issue

  • 20 / 1

Start / End Page

  • 280 - 282

PubMed ID

  • 19942433

Pubmed Central ID

  • PMC3688063

Electronic International Standard Serial Number (EISSN)

  • 1464-3405

International Standard Serial Number (ISSN)

  • 0960-894X

Digital Object Identifier (DOI)

  • 10.1016/j.bmcl.2009.10.114


  • eng