A single step purification for autolytic zinc proteinases.

Journal Article (Journal Article)

We describe a novel single-step method for the purification of stromelysin-1 catalytic domain (SCD) via immobilized metal affinity chromatography under denaturing conditions that inhibit proteolytic activity followed by on-column refolding and spontaneous autolysis of the fusion peptide to yield pure, active stromelysin-1 catalytic domain. The methodology provides a general approach for the rapid purification of large quantities of zinc proteinases.

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Pubmed Central version

Duke Authors

Toone, Eric John

Cited Authors

Wilfong, EM; Locklear, U; Toone, EJ

Published Date

January 2010

Published In

Bioorganic & Medicinal Chemistry Letters

Volume / Issue

20 / 1

Start / End Page

280 - 282

PubMed ID

19942433

Pubmed Central ID

PMC3688063

Electronic International Standard Serial Number (EISSN)

1464-3405

International Standard Serial Number (ISSN)

0960-894X

Digital Object Identifier (DOI)

10.1016/j.bmcl.2009.10.114

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