Singularity in polarization: rewiring yeast cells to make two buds.

Journal Article (Journal Article)

For budding yeast to ensure formation of only one bud, cells must polarize toward one, and only one, site. Polarity establishment involves the Rho family GTPase Cdc42, which concentrates at polarization sites via a positive feedback loop. To assess whether singularity is linked to the specific Cdc42 feedback loop, we disabled the yeast cell's endogenous amplification mechanism and synthetically rewired the cells to employ a different positive feedback loop. Rewired cells violated singularity, occasionally making two buds. Even cells that made only one bud sometimes initiated two clusters of Cdc42, but then one cluster became dominant. Mathematical modeling indicated that, given sufficient time, competition between clusters would promote singularity. In rewired cells, competition occurred slowly and sometimes failed to develop a single "winning" cluster before budding. Slowing competition in normal cells also allowed occasional formation of two buds, suggesting that singularity is enforced by rapid competition between Cdc42 clusters.

Full Text

Duke Authors

Cited Authors

  • Howell, AS; Savage, NS; Johnson, SA; Bose, I; Wagner, AW; Zyla, TR; Nijhout, HF; Reed, MC; Goryachev, AB; Lew, DJ

Published Date

  • November 13, 2009

Published In

Volume / Issue

  • 139 / 4

Start / End Page

  • 731 - 743

PubMed ID

  • 19914166

Pubmed Central ID

  • PMC2783644

Electronic International Standard Serial Number (EISSN)

  • 1097-4172

Digital Object Identifier (DOI)

  • 10.1016/j.cell.2009.10.024


  • eng

Conference Location

  • United States