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Development of magnetic resonance imaging contrast material for in vivo mapping of tissue transglutaminase activity.

Publication ,  Journal Article
Mazooz, G; Mehlman, T; Lai, T-S; Greenberg, CS; Dewhirst, MW; Neeman, M
Published in: Cancer Res
February 15, 2005

Transglutaminases are a family of enzymes that play an important role in tissue remodeling by catalyzing covalent cross-links between proteins of the extracellular matrix. Elevated activity of transglutaminase was shown at the boundaries of invading tumors, in association with angiogenesis, in stabilization of atherosclerotic plaques, and in generation of blood clots. The aim of this work was to develop a low molecular weight substrate of transglutaminase that could serve for noninvasive magnetic resonance and optical mapping of transglutaminase-mediated cross-linking activity. A 2 kDa contrast material was generated which showed cross-linking by either tissue transglutaminase or factor XIII in the context of multicellular tumor spheroids or fibrin clots, respectively. Successful detection by nuclear magnetic resonance microscopy of transglutaminase-mediated cross-linking of the contrast material to MCF7 multicellular spheroids provides hope that this approach could potentially be developed for clinical demarcation of sites of transglutaminase activity.

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Published In

Cancer Res

DOI

ISSN

0008-5472

Publication Date

February 15, 2005

Volume

65

Issue

4

Start / End Page

1369 / 1375

Location

United States

Related Subject Headings

  • alpha-2-Antiplasmin
  • Transglutaminases
  • Substrate Specificity
  • Spheroids, Cellular
  • Peptide Fragments
  • Oncology & Carcinogenesis
  • Oligopeptides
  • Nuclear Magnetic Resonance, Biomolecular
  • Molecular Weight
  • Magnetic Resonance Imaging
 

Citation

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ICMJE
MLA
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Mazooz, G., Mehlman, T., Lai, T.-S., Greenberg, C. S., Dewhirst, M. W., & Neeman, M. (2005). Development of magnetic resonance imaging contrast material for in vivo mapping of tissue transglutaminase activity. Cancer Res, 65(4), 1369–1375. https://doi.org/10.1158/0008-5472.CAN-04-2269
Mazooz, Galit, Tevie Mehlman, Thung-Shen Lai, Charles S. Greenberg, Mark W. Dewhirst, and Michal Neeman. “Development of magnetic resonance imaging contrast material for in vivo mapping of tissue transglutaminase activity.Cancer Res 65, no. 4 (February 15, 2005): 1369–75. https://doi.org/10.1158/0008-5472.CAN-04-2269.
Mazooz G, Mehlman T, Lai T-S, Greenberg CS, Dewhirst MW, Neeman M. Development of magnetic resonance imaging contrast material for in vivo mapping of tissue transglutaminase activity. Cancer Res. 2005 Feb 15;65(4):1369–75.
Mazooz, Galit, et al. “Development of magnetic resonance imaging contrast material for in vivo mapping of tissue transglutaminase activity.Cancer Res, vol. 65, no. 4, Feb. 2005, pp. 1369–75. Pubmed, doi:10.1158/0008-5472.CAN-04-2269.
Mazooz G, Mehlman T, Lai T-S, Greenberg CS, Dewhirst MW, Neeman M. Development of magnetic resonance imaging contrast material for in vivo mapping of tissue transglutaminase activity. Cancer Res. 2005 Feb 15;65(4):1369–1375.

Published In

Cancer Res

DOI

ISSN

0008-5472

Publication Date

February 15, 2005

Volume

65

Issue

4

Start / End Page

1369 / 1375

Location

United States

Related Subject Headings

  • alpha-2-Antiplasmin
  • Transglutaminases
  • Substrate Specificity
  • Spheroids, Cellular
  • Peptide Fragments
  • Oncology & Carcinogenesis
  • Oligopeptides
  • Nuclear Magnetic Resonance, Biomolecular
  • Molecular Weight
  • Magnetic Resonance Imaging