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Characterization of a recombinant adenovirus vector encoding heat-inducible feline interleukin-12 for use in hyperthermia-induced gene-therapy.

Publication ,  Journal Article
Siddiqui, F; Li, C-Y; Zhang, X; Larue, SM; Dewhirst, MW; Ullrich, RL; Avery, PR
Published in: Int J Hyperthermia
March 2006

Interleukin-12 (IL-12) is a pro-inflammatory cytokine that has shown great promise as a therapeutic agent in experimental models of infectious disease and cancer. However, it is also a highly toxic molecule and for that reason has not been accepted readily into the clinic. A replication-deficient adenoviral vector was designed to deliver the feline interleukin-12 gene into tumour cells. The interleukin-12 gene has been placed under control of a heat inducible promoter, human heat shock promoter 70b, with the intent of spatially and temporally controlling the expression of IL-12, thus limiting its toxicity. In vitro, the transfection efficiency of the adenoviral vector, the effect of multiplicity of infection and the production of biologically active feline IL-12 were studied in the infected cells in response to a range of temperatures. This adenoviral vector will be a useful tool to examine the effects of intra-tumoural IL-12 delivery in a spontaneously occurring feline soft tissue sarcoma model.

Duke Scholars

Published In

Int J Hyperthermia

DOI

ISSN

0265-6736

Publication Date

March 2006

Volume

22

Issue

2

Start / End Page

117 / 134

Location

England

Related Subject Headings

  • Transgenes
  • Transfection
  • Temperature
  • Sarcoma
  • RNA, Messenger
  • Promoter Regions, Genetic
  • Oncology & Carcinogenesis
  • Leukocytes, Mononuclear
  • Interleukin-12
  • Interferon-gamma
 

Citation

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Siddiqui, F., Li, C.-Y., Zhang, X., Larue, S. M., Dewhirst, M. W., Ullrich, R. L., & Avery, P. R. (2006). Characterization of a recombinant adenovirus vector encoding heat-inducible feline interleukin-12 for use in hyperthermia-induced gene-therapy. Int J Hyperthermia, 22(2), 117–134. https://doi.org/10.1080/02656730500462309
Siddiqui, Farzan, Chuan-Yuan Li, Xiuwu Zhang, Susan M. Larue, Mark W. Dewhirst, Robert L. Ullrich, and Paul R. Avery. “Characterization of a recombinant adenovirus vector encoding heat-inducible feline interleukin-12 for use in hyperthermia-induced gene-therapy.Int J Hyperthermia 22, no. 2 (March 2006): 117–34. https://doi.org/10.1080/02656730500462309.
Siddiqui F, Li C-Y, Zhang X, Larue SM, Dewhirst MW, Ullrich RL, et al. Characterization of a recombinant adenovirus vector encoding heat-inducible feline interleukin-12 for use in hyperthermia-induced gene-therapy. Int J Hyperthermia. 2006 Mar;22(2):117–34.
Siddiqui, Farzan, et al. “Characterization of a recombinant adenovirus vector encoding heat-inducible feline interleukin-12 for use in hyperthermia-induced gene-therapy.Int J Hyperthermia, vol. 22, no. 2, Mar. 2006, pp. 117–34. Pubmed, doi:10.1080/02656730500462309.
Siddiqui F, Li C-Y, Zhang X, Larue SM, Dewhirst MW, Ullrich RL, Avery PR. Characterization of a recombinant adenovirus vector encoding heat-inducible feline interleukin-12 for use in hyperthermia-induced gene-therapy. Int J Hyperthermia. 2006 Mar;22(2):117–134.
Journal cover image

Published In

Int J Hyperthermia

DOI

ISSN

0265-6736

Publication Date

March 2006

Volume

22

Issue

2

Start / End Page

117 / 134

Location

England

Related Subject Headings

  • Transgenes
  • Transfection
  • Temperature
  • Sarcoma
  • RNA, Messenger
  • Promoter Regions, Genetic
  • Oncology & Carcinogenesis
  • Leukocytes, Mononuclear
  • Interleukin-12
  • Interferon-gamma