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Requirements for T lymphocyte migration in explanted lymph nodes.

Publication ,  Journal Article
Huang, JH; Cárdenas-Navia, LI; Caldwell, CC; Plumb, TJ; Radu, CG; Rocha, PN; Wilder, T; Bromberg, JS; Cronstein, BN; Sitkovsky, M; Dewhirst, MW ...
Published in: J Immunol
June 15, 2007

Although the requirements for T lymphocyte homing to lymph nodes (LNs) are well studied, much less is known about the requirements for T lymphocyte locomotion within LNs. Imaging of murine T lymphocyte migration in explanted LNs using two-photon laser-scanning fluorescence microscopy provides an opportunity to systematically study these requirements. We have developed a closed system for imaging an intact LN with controlled temperature, oxygenation, and perfusion rate. Naive T lymphocyte locomotion in the deep paracortex of the LN required a perfusion rate of >13 microm/s and a partial pressure of O(2) (pO(2)) of >7.4%. Naive T lymphocyte locomotion in the subcapsular region was 38% slower and had higher turning angles and arrest coefficients than naive T lymphocytes in the deep paracortex. T lymphocyte activation decreased the requirement for pO(2), but also decreased the speed of locomotion in the deep paracortex. Although CCR7(-/-) naive T cells displayed a small reduction in locomotion, systemic treatment with pertussis toxin reduced naive T lymphocyte speed by 59%, indicating a contribution of Galpha(i)-mediated signaling, but involvement of other G protein-coupled receptors besides CCR7. Receptor knockouts or pharmacological inhibition in the adenosine, PG/lipoxygenase, lysophosphatidylcholine, and sphingosine-1-phosphate pathways did not individually alter naive T cell migration. These data implicate pO(2), tissue architecture, and G-protein coupled receptor signaling in regulation of naive T lymphocyte migration in explanted LNs.

Duke Scholars

Published In

J Immunol

DOI

ISSN

0022-1767

Publication Date

June 15, 2007

Volume

178

Issue

12

Start / End Page

7747 / 7755

Location

United States

Related Subject Headings

  • T-Lymphocytes
  • Receptors, Chemokine
  • Receptors, CCR7
  • Receptor, Adenosine A2A
  • Perfusion
  • Partial Pressure
  • Oxygen
  • Mice, Knockout
  • Mice
  • Lymph Nodes
 

Citation

APA
Chicago
ICMJE
MLA
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Huang, J. H., Cárdenas-Navia, L. I., Caldwell, C. C., Plumb, T. J., Radu, C. G., Rocha, P. N., … Dustin, M. L. (2007). Requirements for T lymphocyte migration in explanted lymph nodes. J Immunol, 178(12), 7747–7755. https://doi.org/10.4049/jimmunol.178.12.7747
Huang, Julie H., L Isabel Cárdenas-Navia, Charles C. Caldwell, Troy J. Plumb, Caius G. Radu, Paulo N. Rocha, Tuere Wilder, et al. “Requirements for T lymphocyte migration in explanted lymph nodes.J Immunol 178, no. 12 (June 15, 2007): 7747–55. https://doi.org/10.4049/jimmunol.178.12.7747.
Huang JH, Cárdenas-Navia LI, Caldwell CC, Plumb TJ, Radu CG, Rocha PN, et al. Requirements for T lymphocyte migration in explanted lymph nodes. J Immunol. 2007 Jun 15;178(12):7747–55.
Huang, Julie H., et al. “Requirements for T lymphocyte migration in explanted lymph nodes.J Immunol, vol. 178, no. 12, June 2007, pp. 7747–55. Pubmed, doi:10.4049/jimmunol.178.12.7747.
Huang JH, Cárdenas-Navia LI, Caldwell CC, Plumb TJ, Radu CG, Rocha PN, Wilder T, Bromberg JS, Cronstein BN, Sitkovsky M, Dewhirst MW, Dustin ML. Requirements for T lymphocyte migration in explanted lymph nodes. J Immunol. 2007 Jun 15;178(12):7747–7755.

Published In

J Immunol

DOI

ISSN

0022-1767

Publication Date

June 15, 2007

Volume

178

Issue

12

Start / End Page

7747 / 7755

Location

United States

Related Subject Headings

  • T-Lymphocytes
  • Receptors, Chemokine
  • Receptors, CCR7
  • Receptor, Adenosine A2A
  • Perfusion
  • Partial Pressure
  • Oxygen
  • Mice, Knockout
  • Mice
  • Lymph Nodes