Spectral imaging facilitates visualization and measurements of unstable and abnormal microvascular oxygen transport in tumors.
Abnormal microvasculature contributes to the pathophysiologic microenvironment of tumors. Understanding microvascular tumor oxygen transport is necessary to comprehend the factors that influence tumor biology, physiology, and therapy. Previously, we described an in vivo spectral imaging microscopy system for measurements of microvessel hemoglobin saturation (HbSat). We measure temporal fluctuations and spatial gradients in tumor microvessel oxygenation and identify instances of anastomoses between vessels with significantly different oxygenations. Slow periodic fluctuations in HbSat <0.2 cycles per minute were observed. These measurements are consistent with microelectrode measurements of fluctuating tumor oxygenation. Gradients in HbSat along individual tumor microvessels were measured that were larger in magnitude than normal tissue microvessels. Images were captured of anastomoses of tumor microvessels with diameters
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Related Subject Headings
- Spectrum Analysis
- Sensitivity and Specificity
- Oxygen
- Optics
- Neovascularization, Pathologic
- Microscopy
- Microcirculation
- Mice, Nude
- Mice
- Mammary Neoplasms, Experimental
Citation
Published In
DOI
ISSN
Publication Date
Volume
Issue
Start / End Page
Location
Related Subject Headings
- Spectrum Analysis
- Sensitivity and Specificity
- Oxygen
- Optics
- Neovascularization, Pathologic
- Microscopy
- Microcirculation
- Mice, Nude
- Mice
- Mammary Neoplasms, Experimental