Quantitative optical spectroscopy can identify long-term local tumor control in irradiated murine head and neck xenografts.

Journal Article

Noninvasive and longitudinal monitoring of tumor oxygenation status using quantitative diffuse reflectance spectroscopy is used to test whether a final treatment outcome could be estimated from early optical signatures in a murine model of head and neck cancer when treated with radiation. Implanted tumors in the flank of 23 nude mice are exposed to 39 Gy of radiation, while 11 animals exposed to sham irradiation serve as controls. Diffuse optical reflectance is measured from the tumors at baseline (prior to irradiation) and then serially until 17 days posttreatment. The fastest and greatest increase in baseline-corrected blood oxygen saturation levels are observed from the animals that show complete tumor regression with no recurrence 90 days postirradiation, relative to both untreated and treated animals with local recurrences. These increases in saturation are observed starting 5 days posttreatment and last up to 17 days posttreatment. This preclinical study demonstrates that diffuse reflectance spectroscopy could provide a practical method far more effective than the growth delay assay to prognosticate treatment outcome in solid tumors and may hold significant translational promise.

Full Text

Duke Authors

Cited Authors

  • Vishwanath, K; Klein, D; Chang, K; Schroeder, T; Dewhirst, MW; Ramanujam, N

Published Date

  • September 2009

Published In

Volume / Issue

  • 14 / 5

Start / End Page

  • 054051 -

PubMed ID

  • 19895152

Electronic International Standard Serial Number (EISSN)

  • 1560-2281

Digital Object Identifier (DOI)

  • 10.1117/1.3251013

Language

  • eng

Conference Location

  • United States