Global gene expression of Prochlorococcus ecotypes in response to changes in nitrogen availability.

Journal Article (Journal Article)

Nitrogen (N) often limits biological productivity in the oceanic gyres where Prochlorococcus is the most abundant photosynthetic organism. The Prochlorococcus community is composed of strains, such as MED4 and MIT9313, that have different N utilization capabilities and that belong to ecotypes with different depth distributions. An interstrain comparison of how Prochlorococcus responds to changes in ambient nitrogen is thus central to understanding its ecology. We quantified changes in MED4 and MIT9313 global mRNA expression, chlorophyll fluorescence, and photosystem II photochemical efficiency (Fv/Fm) along a time series of increasing N starvation. In addition, the global expression of both strains growing in ammonium-replete medium was compared to expression during growth on alternative N sources. There were interstrain similarities in N regulation such as the activation of a putative NtcA regulon during N stress. There were also important differences between the strains such as in the expression patterns of carbon metabolism genes, suggesting that the two strains integrate N and C metabolism in fundamentally different ways.

Full Text

Duke Authors

Cited Authors

  • Tolonen, AC; Aach, J; Lindell, D; Johnson, ZI; Rector, T; Steen, R; Church, GM; Chisholm, SW

Published Date

  • January 2006

Published In

Volume / Issue

  • 2 /

Start / End Page

  • 53 -

PubMed ID

  • 17016519

Pubmed Central ID

  • PMC1682016

Electronic International Standard Serial Number (EISSN)

  • 1744-4292

International Standard Serial Number (ISSN)

  • 1744-4292

Digital Object Identifier (DOI)

  • 10.1038/msb4100087


  • eng