Cyclic AMP-regulated exocytosis of Escherichia coli from infected bladder epithelial cells.

Published

Journal Article

The superficial bladder epithelium is a powerful barrier to urine and also serves as a regulator of bladder volume, which is achieved by apical exocytosis of specialized fusiform vesicles during distension of the bladder. We report that type 1 fimbriated uropathogenic Escherichia coli (UPEC) circumvents the bladder barrier by harboring in these Rab27b/CD63-positive and cAMP-regulatable fusiform vesicles within bladder epithelial cells (BECs). Incorporation of UPEC into BEC fusiform compartments enabled bacteria to escape elimination during voiding and to re-emerge in the urine as the bladder distended. Notably, treatment of UPEC-infected mice with a drug that increases intracellular cAMP and induces exocytosis of fusiform vesicles reduced the number of intracellular E. coli.

Full Text

Duke Authors

Cited Authors

  • Bishop, BL; Duncan, MJ; Song, J; Li, G; Zaas, D; Abraham, SN

Published Date

  • May 2007

Published In

Volume / Issue

  • 13 / 5

Start / End Page

  • 625 - 630

PubMed ID

  • 17417648

Pubmed Central ID

  • 17417648

International Standard Serial Number (ISSN)

  • 1078-8956

Digital Object Identifier (DOI)

  • 10.1038/nm1572

Language

  • eng

Conference Location

  • United States