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Apolipoprotein E isoform mediated regulation of nitric oxide release.

Publication ,  Journal Article
Brown, CM; Wright, E; Colton, CA; Sullivan, PM; Laskowitz, DT; Vitek, MP
Published in: Free Radic Biol Med
June 1, 2002

Progressive dysfunction and death of neurons in Alzheimer's dementia is enhanced in patients carrying one or more APOE4 alleles who also display increased presence of oxidative stress markers. Modulation of oxidative stress is a nontraditional and physiologically relevant immunomodulatory function of apolipoprotein E (apoE). Stimulated peritoneal macrophages from APOE-transgenic replacement (APOE-TR) mice expressing only human apoE3 or human apoE4 protein isoforms were utilized as mouse models to investigate the role of apoE protein isoforms and gender in the regulation of oxidative stress. Macrophages from male APOE4/4-TR mice produced significantly higher levels of nitric oxide than from male APOE3/3-TR mice, while macrophages from female APOE3/3-TR and female APOE4/4-TR mice produced the similar levels of nitric oxide. Primary cultures of microglial cells of APOE4 transgenic mice also produced significantly more nitric oxide than microglia from APOE3 transgenic mice. These data suggest a potentially novel mechanism for gender-dependent and apoE isoform-dependent immune responses that parallel the genetic susceptibility of APOE4 carriers for the development of Alzheimer's disease.

Duke Scholars

Published In

Free Radic Biol Med

DOI

ISSN

0891-5849

Publication Date

June 1, 2002

Volume

32

Issue

11

Start / End Page

1071 / 1075

Location

United States

Related Subject Headings

  • Protein Isoforms
  • Peritoneum
  • Nitrites
  • Nitric Oxide
  • Microglia
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Mice
  • Male
  • Macrophages
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Brown, C. M., Wright, E., Colton, C. A., Sullivan, P. M., Laskowitz, D. T., & Vitek, M. P. (2002). Apolipoprotein E isoform mediated regulation of nitric oxide release. Free Radic Biol Med, 32(11), 1071–1075. https://doi.org/10.1016/s0891-5849(02)00803-1
Brown, Candice M., Elizabeth Wright, Carol A. Colton, Patrick M. Sullivan, Daniel T. Laskowitz, and Michael P. Vitek. “Apolipoprotein E isoform mediated regulation of nitric oxide release.Free Radic Biol Med 32, no. 11 (June 1, 2002): 1071–75. https://doi.org/10.1016/s0891-5849(02)00803-1.
Brown CM, Wright E, Colton CA, Sullivan PM, Laskowitz DT, Vitek MP. Apolipoprotein E isoform mediated regulation of nitric oxide release. Free Radic Biol Med. 2002 Jun 1;32(11):1071–5.
Brown, Candice M., et al. “Apolipoprotein E isoform mediated regulation of nitric oxide release.Free Radic Biol Med, vol. 32, no. 11, June 2002, pp. 1071–75. Pubmed, doi:10.1016/s0891-5849(02)00803-1.
Brown CM, Wright E, Colton CA, Sullivan PM, Laskowitz DT, Vitek MP. Apolipoprotein E isoform mediated regulation of nitric oxide release. Free Radic Biol Med. 2002 Jun 1;32(11):1071–1075.
Journal cover image

Published In

Free Radic Biol Med

DOI

ISSN

0891-5849

Publication Date

June 1, 2002

Volume

32

Issue

11

Start / End Page

1071 / 1075

Location

United States

Related Subject Headings

  • Protein Isoforms
  • Peritoneum
  • Nitrites
  • Nitric Oxide
  • Microglia
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Mice
  • Male
  • Macrophages