Prevalence and characterization of metallo-beta-lactamases in clinical isolates of pseudomonas aeruginosa.


Journal Article

The prevalence and the type(s) of metallo-beta-lactamases (MBLs) produced by isolates of Pseudomonas aeruginosa were investigated. During 2001, 506 nonduplicate isolates were obtained from hospitalized patients. Eighty-two strains were selected because of resistance to carbapenems and/or ceftazidime. Screening for MBL production was performed in the latter isolates by the Etest MBL strips (AB Biodisk, Solna, Sweden) and by a broth microdilution method measuring minimum inhibitory concentrations (MICs) of imipenem alone and in the presence of metal-chelating agents (EDTA and o-phenanthroline). Specific DNA probes were used to investigate the presence of genes coding for IMP- or VIM-type enzymes. Overall, four isolates of P. aeruginosa (obtained from independent patients) were found to carry a blaVIM gene. Polymerase chain reaction (PCR) experiments and DNA sequencing revealed that the VIM-2 determinant was present in three cases, whereas VIM-1 was detected in one isolate. Surveillance programs should be adopted to avoid the spread of these worrisome resistance genes.

Full Text

Duke Authors

Cited Authors

  • Luzzaro, F; Endimiani, A; Docquier, J-D; Mugnaioli, C; Bonsignori, M; Amicosante, G; Rossolini, GM; Toniolo, A

Published Date

  • February 2004

Published In

Volume / Issue

  • 48 / 2

Start / End Page

  • 131 - 135

PubMed ID

  • 14972383

Pubmed Central ID

  • 14972383

International Standard Serial Number (ISSN)

  • 0732-8893

Digital Object Identifier (DOI)

  • 10.1016/j.diagmicrobio.2003.09.005


  • eng

Conference Location

  • United States