Proteomic analysis of S-nitrosylation and denitrosylation by resin-assisted capture.
Published
Journal Article
We have modified the biotin switch assay for protein S-nitrosothiols (SNOs), using resin-assisted capture (SNO-RAC). Compared with existing methodologies, SNO-RAC requires fewer steps, detects high-mass S-nitrosylated proteins more efficiently, and facilitates identification and quantification of S-nitrosylated sites by mass spectrometry. When combined with iTRAQ labeling, SNO-RAC revealed that intracellular proteins may undergo rapid denitrosylation on a global scale. This methodology is readily adapted to analyzing diverse cysteine-based protein modifications, including S-acylation.
Full Text
Duke Authors
Cited Authors
- Forrester, MT; Thompson, JW; Foster, MW; Nogueira, L; Moseley, MA; Stamler, JS
Published Date
- June 2009
Published In
Volume / Issue
- 27 / 6
Start / End Page
- 557 - 559
PubMed ID
- 19483679
Pubmed Central ID
- 19483679
Electronic International Standard Serial Number (EISSN)
- 1546-1696
Digital Object Identifier (DOI)
- 10.1038/nbt.1545
Language
- eng
Conference Location
- United States