Proteomic analysis of S-nitrosylation and denitrosylation by resin-assisted capture.

Journal Article (Journal Article)

We have modified the biotin switch assay for protein S-nitrosothiols (SNOs), using resin-assisted capture (SNO-RAC). Compared with existing methodologies, SNO-RAC requires fewer steps, detects high-mass S-nitrosylated proteins more efficiently, and facilitates identification and quantification of S-nitrosylated sites by mass spectrometry. When combined with iTRAQ labeling, SNO-RAC revealed that intracellular proteins may undergo rapid denitrosylation on a global scale. This methodology is readily adapted to analyzing diverse cysteine-based protein modifications, including S-acylation.

Full Text

Duke Authors

Cited Authors

  • Forrester, MT; Thompson, JW; Foster, MW; Nogueira, L; Moseley, MA; Stamler, JS

Published Date

  • June 2009

Published In

Volume / Issue

  • 27 / 6

Start / End Page

  • 557 - 559

PubMed ID

  • 19483679

Pubmed Central ID

  • PMC2891235

Electronic International Standard Serial Number (EISSN)

  • 1546-1696

Digital Object Identifier (DOI)

  • 10.1038/nbt.1545

Language

  • eng

Conference Location

  • United States