Detection of protein S-nitrosylation with the biotin-switch technique.

Journal Article (Journal Article;Review)

Protein S-nitrosylation, the posttranslational modification of cysteine thiols to form S-nitrosothiols, is a principle mechanism of nitric oxide-based signaling. Studies have demonstrated myriad roles for S-nitrosylation in organisms from bacteria to humans, and recent efforts have greatly advanced our scientific understanding of how this redox-based modification is dynamically regulated during physiological and pathophysiological conditions. The focus of this review is the biotin-switch technique (BST), which has become a mainstay assay for detecting S-nitrosylated proteins in complex biological systems. Potential pitfalls and modern adaptations of the BST are discussed, as are future directions for this assay in the burgeoning field of protein S-nitrosylation.

Full Text

Duke Authors

Cited Authors

  • Forrester, MT; Foster, MW; Benhar, M; Stamler, JS

Published Date

  • January 15, 2009

Published In

Volume / Issue

  • 46 / 2

Start / End Page

  • 119 - 126

PubMed ID

  • 18977293

Pubmed Central ID

  • PMC3120222

Electronic International Standard Serial Number (EISSN)

  • 1873-4596

Digital Object Identifier (DOI)

  • 10.1016/j.freeradbiomed.2008.09.034


  • eng

Conference Location

  • United States