Detection of protein S-nitrosylation with the biotin-switch technique.
Protein S-nitrosylation, the posttranslational modification of cysteine thiols to form S-nitrosothiols, is a principle mechanism of nitric oxide-based signaling. Studies have demonstrated myriad roles for S-nitrosylation in organisms from bacteria to humans, and recent efforts have greatly advanced our scientific understanding of how this redox-based modification is dynamically regulated during physiological and pathophysiological conditions. The focus of this review is the biotin-switch technique (BST), which has become a mainstay assay for detecting S-nitrosylated proteins in complex biological systems. Potential pitfalls and modern adaptations of the BST are discussed, as are future directions for this assay in the burgeoning field of protein S-nitrosylation.
Duke Scholars
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Related Subject Headings
- Sulfhydryl Compounds
- Signal Transduction
- S-Nitrosothiols
- Protein Processing, Post-Translational
- Oxidation-Reduction
- Nitric Oxide
- Humans
- Cysteine
- Chemistry Techniques, Analytical
- Cell Extracts
Citation
Published In
DOI
EISSN
Publication Date
Volume
Issue
Start / End Page
Location
Related Subject Headings
- Sulfhydryl Compounds
- Signal Transduction
- S-Nitrosothiols
- Protein Processing, Post-Translational
- Oxidation-Reduction
- Nitric Oxide
- Humans
- Cysteine
- Chemistry Techniques, Analytical
- Cell Extracts