Phage display screening for peptides that inhibit polyglutamine aggregation.

Journal Article (Journal Article)

Proteins with expanded polyglutamine domains cause nine dominantly inherited, neurodegenerative diseases, including Huntington's disease. There are no therapies that inhibit disease onset or progression. To identify a novel therapeutic, we screened phage displayed peptide libraries for phage that bind preferentially to expanded polyglutamine repeats. We identified a peptide motif that inhibits polyglutamine aggregation in vitro and inhibits death in cellular and Drosophila models of the polyglutamine repeat diseases. In this chapter, we describe in detail how to screen a peptide phage display library and highlight results demonstrating the success of this approach. A similar experimental approach could be used for other diseases caused by conformational change in disease proteins, including prion, Alzheimer's, and Parkinson's diseases.

Full Text

Duke Authors

Cited Authors

  • Kenan, DJ; Strittmatter, WJ; Burke, JR

Published Date

  • 2006

Published In

Volume / Issue

  • 413 /

Start / End Page

  • 253 - 273

PubMed ID

  • 17046401

International Standard Serial Number (ISSN)

  • 0076-6879

Digital Object Identifier (DOI)

  • 10.1016/S0076-6879(06)13014-1


  • eng

Conference Location

  • United States