Rapid detection of anti-chromatin autoantibodies in human serum using a portable electrochemical biosensor

The current system employed electrochemical sensor technology for the measurement of autoantibodies in human sera. Anti-chromatin antibodies are an early and sensitive indicator of systemic lupus erythematosus (SLE) and are typically quantified using enzyme-linked immunosorbent assays (ELISA). Electrochemical detection compared favorably with ELISA using a sample of 30 SLE sera (r = 0.9), and non-specific binding by normal serum immunoglobulin was undetectable. The electrochemical sensor assay required <20 min processing time and utilized a hand-held apparatus with a disposable electrode. These results demonstrate the applicability of this technology to the rapid measurement of a clinically relevant analyte with an apparatus of potential simplicity and low cost. © 2008 Elsevier B.V. All rights reserved.

Full Text

Duke Authors

Cited Authors

  • Konstantinov, KN; Sitdikov, RA; Lopez, GP; Atanassov, P; Rubin, RL

Published Date

  • 2009

Published In

Volume / Issue

  • 24 / 7

Start / End Page

  • 1949 - 1954

International Standard Serial Number (ISSN)

  • 0956-5663

Digital Object Identifier (DOI)

  • 10.1016/j.bios.2008.09.032