Localization of cloned unique DNA to three different regions of chromosome 19: screen for linkage probes for myotonic dystrophy.
Journal Article
Screening polymorphic DNA probes for linkage to myotonic dystrophy (DM) and to other reported chromosome 19 (CH19) genes will develop a linkage map for human CH19. We report here the assignment of 3 cloned unique DNA sequences to 3 distinct regions of CH19. The novel use of 35S-labeled probes facilitated the rapid localization of the gene for the third complement factor (C3) to 19p13.2 by in situ hybridization. Metaphase chromosomes were from normal peripheral lymphocytes as well as from a fibroblast line containing a 15;19 translocation which permitted clear identification of CH19 regions of localization. Two random clones isolated from a plasmid library of human F-group enriched chromosomal DNA (D19S5 and D19S6) were in like manner assigned to 19p1.2 and 19q13.2 to 19qter, respectively.
Full Text
Duke Authors
Cited Authors
- Yamaoka, LH; Bartlett, RJ; Ross, DA; Fey, GH; Ledbetter, DH; Bruns, G; Pericak-Vance, MA; Herbstreith, MH; Roses, AD
Published Date
- December 1985
Published In
Volume / Issue
- 2 / 6
Start / End Page
- 403 - 412
PubMed ID
- 3001264
Pubmed Central ID
- 3001264
International Standard Serial Number (ISSN)
- 0167-7063
Digital Object Identifier (DOI)
- 10.3109/01677068509101426
Language
- eng
Conference Location
- England