Localization of cloned unique DNA to three different regions of chromosome 19: screen for linkage probes for myotonic dystrophy.
Screening polymorphic DNA probes for linkage to myotonic dystrophy (DM) and to other reported chromosome 19 (CH19) genes will develop a linkage map for human CH19. We report here the assignment of 3 cloned unique DNA sequences to 3 distinct regions of CH19. The novel use of 35S-labeled probes facilitated the rapid localization of the gene for the third complement factor (C3) to 19p13.2 by in situ hybridization. Metaphase chromosomes were from normal peripheral lymphocytes as well as from a fibroblast line containing a 15;19 translocation which permitted clear identification of CH19 regions of localization. Two random clones isolated from a plasmid library of human F-group enriched chromosomal DNA (D19S5 and D19S6) were in like manner assigned to 19p1.2 and 19q13.2 to 19qter, respectively.
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- Polymorphism, Genetic
- Nucleic Acid Hybridization
- Neurology & Neurosurgery
- Myotonic Dystrophy
- Humans
- Genetic Linkage
- DNA Restriction Enzymes
- Complement C3
- Cloning, Molecular
- Chromosomes, Human, 19-20
Citation
Published In
DOI
ISSN
Publication Date
Volume
Issue
Start / End Page
Location
Related Subject Headings
- Polymorphism, Genetic
- Nucleic Acid Hybridization
- Neurology & Neurosurgery
- Myotonic Dystrophy
- Humans
- Genetic Linkage
- DNA Restriction Enzymes
- Complement C3
- Cloning, Molecular
- Chromosomes, Human, 19-20