Isoform-specific interactions of apolipoprotein E with the microtubule-associated protein MAP2c: implications for Alzheimer's disease.

Published

Journal Article

The apolipoprotein E type 4 allele is a susceptibility gene for late-onset Alzheimer's disease. Apolipoprotein E is found in neurons, some of which contain paired helical filaments made of the microtubule-associated protein tau. Previous studies have demonstrated that the apoE3 isoform, but not the apoE4 isoform, binds tau with high avidity. Because the microtubule-associated protein MAP2c also effects microtubule assembly and stability, we examined interactions between apoE isoforms and MAP2c. Similar to the tau-binding results, apoE3, but not apoE4, bound MAP2c. Binding was detectable down to 10(-9) M MAP2c and 10(-8) M apoE3. Isoform-specific interactions of apoE with the microtubule-associated proteins MAP2c and tau might affect intracellular maintenance of microtubules and could contribute to a time-dependent pathogenesis of Alzheimer's disease.

Full Text

Duke Authors

Cited Authors

  • Huang, DY; Goedert, M; Jakes, R; Weisgraber, KH; Garner, CC; Saunders, AM; Pericak-Vance, MA; Schmechel, DE; Roses, AD; Strittmatter, WJ

Published Date

  • November 1994

Published In

Volume / Issue

  • 182 / 1

Start / End Page

  • 55 - 58

PubMed ID

  • 7891887

Pubmed Central ID

  • 7891887

Electronic International Standard Serial Number (EISSN)

  • 1872-7972

International Standard Serial Number (ISSN)

  • 0304-3940

Digital Object Identifier (DOI)

  • 10.1016/0304-3940(94)90204-6

Language

  • eng