Isoform-specific interactions of apolipoprotein E with the microtubule-associated protein MAP2c: implications for Alzheimer's disease.
Journal Article
The apolipoprotein E type 4 allele is a susceptibility gene for late-onset Alzheimer's disease. Apolipoprotein E is found in neurons, some of which contain paired helical filaments made of the microtubule-associated protein tau. Previous studies have demonstrated that the apoE3 isoform, but not the apoE4 isoform, binds tau with high avidity. Because the microtubule-associated protein MAP2c also effects microtubule assembly and stability, we examined interactions between apoE isoforms and MAP2c. Similar to the tau-binding results, apoE3, but not apoE4, bound MAP2c. Binding was detectable down to 10(-9) M MAP2c and 10(-8) M apoE3. Isoform-specific interactions of apoE with the microtubule-associated proteins MAP2c and tau might affect intracellular maintenance of microtubules and could contribute to a time-dependent pathogenesis of Alzheimer's disease.
Full Text
Duke Authors
Cited Authors
- Huang, DY; Goedert, M; Jakes, R; Weisgraber, KH; Garner, CC; Saunders, AM; Pericak-Vance, MA; Schmechel, DE; Roses, AD; Strittmatter, WJ
Published Date
- November 21, 1994
Published In
Volume / Issue
- 182 / 1
Start / End Page
- 55 - 58
PubMed ID
- 7891887
Pubmed Central ID
- 7891887
International Standard Serial Number (ISSN)
- 0304-3940
Digital Object Identifier (DOI)
- 10.1016/0304-3940(94)90204-6
Language
- eng
Conference Location
- Ireland