Relative abundance of Alzheimer A beta amyloid peptide variants in Alzheimer disease and normal aging.


Journal Article

The Alzheimer A beta amyloid peptide (A beta) is the principal proteinaceous component of amyloid associated with Alzheimer disease (AD). We have determined the relative abundance of A beta structural variants present in amyloid from brains of 10 individuals with sporadic AD, 2 individuals with familial AD carrying specific mutations in the Alzheimer amyloid precursor protein gene, and 5 nondemented elderly controls. A procedure of isolation based on the extreme insolubility of A beta amyloid was used. The purified, nondigested A beta was analyzed by N-terminal sequencing and electrospray-ionization mass spectrometry. Three principal A beta variants were detected--A beta-(1-40), A beta-(1-42), and A beta-(11-42)--in all brains analyzed. The predominant variant in sporadic AD was A beta-(1-40), whereas the principal A beta variant in nondemented elderly controls was A beta-(1-42). The ratio A beta-(1-40)/A beta-(1-42) differed by 10-fold between brains from nondemented controls and those with sporadic AD.

Full Text

Cited Authors

  • Näslund, J; Schierhorn, A; Hellman, U; Lannfelt, L; Roses, AD; Tjernberg, LO; Silberring, J; Gandy, SE; Winblad, B; Greengard, P

Published Date

  • August 1994

Published In

Volume / Issue

  • 91 / 18

Start / End Page

  • 8378 - 8382

PubMed ID

  • 8078890

Pubmed Central ID

  • 8078890

Electronic International Standard Serial Number (EISSN)

  • 1091-6490

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.91.18.8378


  • eng