Human apolipoprotein E isoform-specific differences in neuronal sprouting in organotypic hippocampal culture.


Journal Article

The apolipoprotein E (ApoE) epsilon4 allele is a major risk factor for neurodegenerative conditions, including Alzheimer's disease. A role for ApoE is implicated in regeneration of synaptic circuitry after neural injury. In the in vitro mouse organotypic hippocampal slice culture system, we previously showed that cultures derived from ApoE-knockout mice are defective in mossy fiber sprouting into the dentate gyrus molecular layer. This sprouting defect was rescued in cultures from transgenic mice expressing ApoE3 under the control of the human promoter and in ApoE-knockout cultures treated with ApoE3-conditioned media. Although the ApoE3 transgene fully restored sprouting, ApoE4 restored sprouting to only 58% of ApoE3 levels. These data indicate that ApoE isoform-specific effects on neuroregeneration may contribute to its genetic risk for Alzheimer's disease.

Full Text

Cited Authors

  • Teter, B; Xu, PT; Gilbert, JR; Roses, AD; Galasko, D; Cole, GM

Published Date

  • December 1999

Published In

Volume / Issue

  • 73 / 6

Start / End Page

  • 2613 - 2616

PubMed ID

  • 10582625

Pubmed Central ID

  • 10582625

Electronic International Standard Serial Number (EISSN)

  • 1471-4159

International Standard Serial Number (ISSN)

  • 0022-3042

Digital Object Identifier (DOI)

  • 10.1046/j.1471-4159.1999.0732613.x


  • eng