Characterization of diffusing capacity and perfusion of the rat lung in a lipopolysaccaride disease model using hyperpolarized 129Xe.

Journal Article (Journal Article)

The ability to quantify pulmonary diffusing capacity and perfusion using dynamic hyperpolarized (129)Xe NMR spectroscopy is demonstrated. A model of alveolar gas exchange was developed, which, in conjunction with (129)Xe NMR, enables quantification of average alveolar wall thickness, pulmonary perfusion, capillary diffusion length, and mean transit time. The technique was employed to compare a group of naïve rats (n = 10) with a group of rats with acute inflammatory lung injury (n = 10), caused by instillation of lipopolysaccaride (LPS). The measured structural and perfusion-related parameters were in agreement with reported values from studies using non-NMR methods. Significant differences between the groups were found in total diffusion length (control 8.5 +/- 0.5 microm, LPS 9.9 +/- 0.6 microm, P < 0.001), in capillary diffusion length (control 2.9 +/- 0.4 microm, LPS 3.9 +/- 1.0 microm, P < 0.05), and in pulmonary hematocrit (control 0.55 +/- 0.06, LPS 0.43 +/- 0.08, P < 0.01), whereas no differences were observed in alveolar wall thickness, pulmonary perfusion, and mean transit time. These results demonstrate the ability of the method to distinguish two main aspects of lung function, namely, diffusing capacity and pulmonary perfusion.

Full Text

Duke Authors

Cited Authors

  • Månsson, S; Wolber, J; Driehuys, B; Wollmer, P; Golman, K

Published Date

  • December 2003

Published In

Volume / Issue

  • 50 / 6

Start / End Page

  • 1170 - 1179

PubMed ID

  • 14648564

International Standard Serial Number (ISSN)

  • 0740-3194

Digital Object Identifier (DOI)

  • 10.1002/mrm.10649


  • eng

Conference Location

  • United States