Noninvasive monitoring of tissue hemoglobin using UV-VIS diffuse reflectance spectroscopy: a pilot study.

Journal Article (Journal Article)

We conducted a pilot study on 10 patients undergoing general surgery to test the feasibility of diffuse reflectance spectroscopy in the visible wavelength range as a noninvasive monitoring tool for blood loss during surgery. Ratios of raw diffuse reflectance at wavelength pairs were tested as a first-pass for estimating hemoglobin concentration. Ratios can be calculated easily and rapidly with limited post-processing, and so this can be considered a near real-time monitoring device. We found the best hemoglobin correlations were when ratios at isosbestic points of oxy- and deoxyhemoglobin were used, specifically 529/500 nm. Baseline subtraction improved correlations, specifically at 520/509 nm. These results demonstrate proof-of-concept for the ability of this noninvasive device to monitor hemoglobin concentration changes due to surgical blood loss. The 529/500 nm ratio also appears to account for variations in probe pressure, as determined from measurements on two volunteers.

Full Text

Duke Authors

Cited Authors

  • Bender, JE; Shang, AB; Moretti, EW; Yu, B; Richards, LM; Ramanujam, N

Published Date

  • December 21, 2009

Published In

Volume / Issue

  • 17 / 26

Start / End Page

  • 23396 - 23409

PubMed ID

  • 20052047

Electronic International Standard Serial Number (EISSN)

  • 1094-4087

Digital Object Identifier (DOI)

  • 10.1364/OE.17.023396


  • eng

Conference Location

  • United States