A robust Monte Carlo model for the extraction of biological absorption and scattering in vivo.

Journal Article (Journal Article)

We have a toolbox to quantify tissue optical properties that is composed of specialized fiberoptic probes for UV-visible diffuse reflectance spectroscopy and a fast, scalable inverse Monte Carlo (MC) model. In this paper, we assess the robustness of the toolbox for quantifying physiologically relevant parameters from turbid tissue-like media. In particular, we consider the effects of using different instruments, fiberoptic probes, and instrument-specific settings for a wide range of optical properties. Additionally, we test the quantitative accuracy of the inverse MC model for extracting the biologically relevant parameters of hemoglobin saturation and total hemoglobin concentration. We also test the effect of double-absorber phantoms (hemoglobin and crocin to model the absorption of hemoglobin and beta carotene, respectively, in the breast) for a range of absorption and scattering properties. We include an assessment on which reference phantom serves as the best calibration standard to enable accurate extraction of the absorption and scattering properties of the target sample. We found the best reference-target phantom combinations to be ones with similar scattering levels. The results from these phantom studies provide a set of guidelines for extracting optical parameters from clinical studies.

Full Text

Duke Authors

Cited Authors

  • Bender, JE; Vishwanath, K; Moore, LK; Brown, JQ; Chang, V; Palmer, GM; Ramanujam, N

Published Date

  • April 2009

Published In

Volume / Issue

  • 56 / 4

Start / End Page

  • 960 - 968

PubMed ID

  • 19423425

Pubmed Central ID

  • PMC2791541

Electronic International Standard Serial Number (EISSN)

  • 1558-2531

Digital Object Identifier (DOI)

  • 10.1109/TBME.2008.2005994


  • eng

Conference Location

  • United States