Quantitative diffuse reflectance and fluorescence spectroscopy: tool to monitor tumor physiology in vivo.

Journal Article

This study demonstrates the use of optical spectroscopy for monitoring tumor oxygenation and metabolism in response to hyperoxic gas breathing. Hemoglobin saturation and redox ratio were quantified for a set of 14 and 9 mice, respectively, measured at baseline and during carbogen breathing (95% O(2), 5% CO(2)). In particular, significant increases in hemoglobin saturation and fluorescence redox ratio were observed upon carbogen breathing. These data were compared with data obtained concurrently using an established invasive technique, the OxyLite partial oxygen pressure (pO(2)) system, which also showed a significant increase in pO(2). It was found that the direction of changes were generally the same between all of the methods, but that the OxyLite system was much more variable in general, suggesting that optical techniques may provide a better assessment of global tumor physiology. Optical spectroscopy measurements are demonstrated to provide a reliable, reproducible indication of changes in tumor physiology in response to physiologic manipulation.

Full Text

Duke Authors

Cited Authors

  • Palmer, GM; Viola, RJ; Schroeder, T; Yarmolenko, PS; Dewhirst, MW; Ramanujam, N

Published Date

  • March 2009

Published In

Volume / Issue

  • 14 / 2

Start / End Page

  • 024010 -

PubMed ID

  • 19405740

International Standard Serial Number (ISSN)

  • 1083-3668

Digital Object Identifier (DOI)

  • 10.1117/1.3103586

Language

  • eng

Conference Location

  • United States