Identification of calcium oxalate crystals using alizarin red S stain.

Journal Article (Journal Article)

Calcium oxalate crystals stain with alizarin red S at a pH of 7.0 but not at a pH of 4.2. In contrast, calcium phosphate and calcium carbonate stain at a pH of both 7.0 and 4.2. This difference allows presumptive identification of calcium oxalate deposits. The identity of calcium oxalate can then be confirmed by its insolubility in 2M acetic acid, since both calcium carbonate and calcium phosphate are soluble. We have applied this procedure for several years and have found it to be a rapid, reliable, and technically simple procedure for distinguishing calcium oxalate from other calcium deposits.

Full Text

Duke Authors

Cited Authors

  • Proia, AD; Brinn, NT

Published Date

  • February 1985

Published In

Volume / Issue

  • 109 / 2

Start / End Page

  • 186 - 189

PubMed ID

  • 2579619

International Standard Serial Number (ISSN)

  • 0003-9985


  • eng

Conference Location

  • United States