Deoxycholate induces the preferential hydrolysis of polyphosphoinositides by human platelet and rat corneal phospholipase C.

Journal Article (Journal Article)

Deoxycholate promotes phospholipase C degradation of endogenous phosphatidyl[3H]inositol (Pl), phosphatidyl[3H]inositol monophosphate (PIP) and phosphatidyl[3H]inositol bisphosphate (PIP2) in rat cornea and human platelets. Hydrolysis of phosphatidyl[3H]inositol significantly lags polyphospho[3H]inositide degradation. Concomitantly, formation of [3H]inositol monophosphate (IP1) lags behind [3H]inositol bisphosphate (IP2) and [3H]inositol trisphosphate (IP3) production. These results demonstrate that rat cornea and human platelet phospholipase C cause a preferential hydrolysis of the endogenous polyphosphoinositides rather than phosphatidylinositol.

Full Text

Duke Authors

Cited Authors

  • Chung, SM; Proia, AD; Klintworth, GK; Watson, SP; Lapetina, EG

Published Date

  • June 14, 1985

Published In

Volume / Issue

  • 129 / 2

Start / End Page

  • 411 - 416

PubMed ID

  • 2990455

International Standard Serial Number (ISSN)

  • 0006-291X

Digital Object Identifier (DOI)

  • 10.1016/0006-291x(85)90166-4

Language

  • eng

Conference Location

  • United States