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The histology of graft adhesion in descemet stripping with endothelial keratoplasty.

Publication ,  Journal Article
Caldwell, MC; Afshari, NA; Decroos, FC; Proia, AD
Published in: Am J Ophthalmol
August 2009

PURPOSE: To describe the histologic findings of full-thickness corneas from penetrating keratoplasty (PK) in 10 patients with a prior history of Descemet stripping with endothelial keratoplasty. DESIGN: Retrospective review of the histopathology of 10 PK specimens. METHODS: We reviewed histologic sections stained with hematoxylin and eosin, periodic acid-Schiff, and colloidal iron stains from 10 PK specimens, which had been trisected and submitted in their entirety. Clinical data were abstracted from the medical record. RESULTS: The interface between host and graft in most cases was barely perceptible with minimal changes in the degree of tissue eosinophilia, a subtle increase in keratocyte cellularity focally, or the presence of melanin granules within keratocytes. Residual host Descemet membrane (DM) was found in 8 of the 10 cases, most often at the edges of the graft. In no case did the presence of DM appear to hinder graft adhesion. CONCLUSION: The adhesion of Descemet stripping with endothelial keratoplasty grafts is not associated with significant scarring or keratocyte proliferation. Contrary to previous assumptions, retained DM did not appear to hinder graft adhesion, raising the possibility that removal of DM may be unnecessary for endothelial transplantation.

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Published In

Am J Ophthalmol

DOI

EISSN

1879-1891

Publication Date

August 2009

Volume

148

Issue

2

Start / End Page

277 / 281

Location

United States

Related Subject Headings

  • Young Adult
  • Wound Healing
  • Tissue Donors
  • Retrospective Studies
  • Ophthalmology & Optometry
  • Middle Aged
  • Male
  • Keratoplasty, Penetrating
  • Humans
  • Graft Survival
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Caldwell, M. C., Afshari, N. A., Decroos, F. C., & Proia, A. D. (2009). The histology of graft adhesion in descemet stripping with endothelial keratoplasty. Am J Ophthalmol, 148(2), 277–281. https://doi.org/10.1016/j.ajo.2009.03.025
Caldwell, Matthew C., Natalie A. Afshari, Francis C. Decroos, and Alan D. Proia. “The histology of graft adhesion in descemet stripping with endothelial keratoplasty.Am J Ophthalmol 148, no. 2 (August 2009): 277–81. https://doi.org/10.1016/j.ajo.2009.03.025.
Caldwell MC, Afshari NA, Decroos FC, Proia AD. The histology of graft adhesion in descemet stripping with endothelial keratoplasty. Am J Ophthalmol. 2009 Aug;148(2):277–81.
Caldwell, Matthew C., et al. “The histology of graft adhesion in descemet stripping with endothelial keratoplasty.Am J Ophthalmol, vol. 148, no. 2, Aug. 2009, pp. 277–81. Pubmed, doi:10.1016/j.ajo.2009.03.025.
Caldwell MC, Afshari NA, Decroos FC, Proia AD. The histology of graft adhesion in descemet stripping with endothelial keratoplasty. Am J Ophthalmol. 2009 Aug;148(2):277–281.
Journal cover image

Published In

Am J Ophthalmol

DOI

EISSN

1879-1891

Publication Date

August 2009

Volume

148

Issue

2

Start / End Page

277 / 281

Location

United States

Related Subject Headings

  • Young Adult
  • Wound Healing
  • Tissue Donors
  • Retrospective Studies
  • Ophthalmology & Optometry
  • Middle Aged
  • Male
  • Keratoplasty, Penetrating
  • Humans
  • Graft Survival