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Basal-like cells constitute the proliferating cell population in cystic fibrosis airways.

Publication ,  Journal Article
Voynow, JA; Fischer, BM; Roberts, BC; Proia, AD
Published in: Am J Respir Crit Care Med
October 15, 2005

RATIONALE: Cystic fibrosis airways are recurrently exposed to noxious stimuli, leading to epithelial injury. Previous reports suggest that cystic fibrosis airway epithelia may respond to injury by increasing proliferation. OBJECTIVES: We sought to determine the characteristics of the proliferating cell population in cystic fibrosis airways. METHODS: Six cystic fibrosis and six normal lung sections from lung transplant recipients or lung surgery were obtained from the Duke Hospital pathology archives. Sections containing bronchi were evaluated for epithelial cell proliferation using immunohistochemistry for a nuclear proliferation antigen, Ki-67, and image analysis; immunohistochemistry for basal cells using a cytokeratin 5/14 antibody; and immunohistochemistry for the epidermal growth factor receptor and ErbB2, two receptor tyrosine kinases implicated in epithelial proliferation and differentiation. RESULTS: Overall, cystic fibrosis sections had a greater proliferation index than control sections with 25.1 +/- 2.1% positively staining nuclei/total nuclei compared with control sections, 4.6 +/- 0.9% (p = 0.002). In cystic fibrosis sections only, there were areas of hyperplastic cuboidal cells adjacent to normal pseudostratified columnar epithelial sections; in these areas of epithelial hyperplasia, there was uniform Ki-67 staining, indicating a zone of proliferating cells. The proliferating cell population also expressed the basal cell cytokeratins 5/14 and epidermal growth factor receptor. Expression of ErbB2 was diminished in the proliferating cells. CONCLUSIONS: Our results suggest that basal-like cells, expressing the epidermal growth factor receptor, constitute the proliferating cell population in cystic fibrosis airways.

Duke Scholars

Published In

Am J Respir Crit Care Med

DOI

ISSN

1073-449X

Publication Date

October 15, 2005

Volume

172

Issue

8

Start / End Page

1013 / 1018

Location

United States

Related Subject Headings

  • Respiratory System
  • Respiratory Mucosa
  • Receptor, erbB-2
  • Receptor, ErbB-2
  • Photomicrography
  • Middle Aged
  • Male
  • Ki-67 Antigen
  • Keratins
  • Infant
 

Citation

APA
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ICMJE
MLA
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Voynow, J. A., Fischer, B. M., Roberts, B. C., & Proia, A. D. (2005). Basal-like cells constitute the proliferating cell population in cystic fibrosis airways. Am J Respir Crit Care Med, 172(8), 1013–1018. https://doi.org/10.1164/rccm.200410-1398OC
Voynow, Judith A., Bernard M. Fischer, Bruce C. Roberts, and Alan D. Proia. “Basal-like cells constitute the proliferating cell population in cystic fibrosis airways.Am J Respir Crit Care Med 172, no. 8 (October 15, 2005): 1013–18. https://doi.org/10.1164/rccm.200410-1398OC.
Voynow JA, Fischer BM, Roberts BC, Proia AD. Basal-like cells constitute the proliferating cell population in cystic fibrosis airways. Am J Respir Crit Care Med. 2005 Oct 15;172(8):1013–8.
Voynow, Judith A., et al. “Basal-like cells constitute the proliferating cell population in cystic fibrosis airways.Am J Respir Crit Care Med, vol. 172, no. 8, Oct. 2005, pp. 1013–18. Pubmed, doi:10.1164/rccm.200410-1398OC.
Voynow JA, Fischer BM, Roberts BC, Proia AD. Basal-like cells constitute the proliferating cell population in cystic fibrosis airways. Am J Respir Crit Care Med. 2005 Oct 15;172(8):1013–1018.

Published In

Am J Respir Crit Care Med

DOI

ISSN

1073-449X

Publication Date

October 15, 2005

Volume

172

Issue

8

Start / End Page

1013 / 1018

Location

United States

Related Subject Headings

  • Respiratory System
  • Respiratory Mucosa
  • Receptor, erbB-2
  • Receptor, ErbB-2
  • Photomicrography
  • Middle Aged
  • Male
  • Ki-67 Antigen
  • Keratins
  • Infant