Inhaled ethyl nitrite prevents hyperoxia-impaired postnatal alveolar development in newborn rats.

Published

Journal Article

RATIONALE: Inhaled nitric oxide (NO) has been used to prevent bronchopulmonary dysplasia, but with variable results. Ethyl nitrite (ENO) forms S-nitrosothiols more readily than does NO, and resists higher-order nitrogen oxide formation. Because S-nitrosylation is a key pathway mediating many NO biological effects, treatment with inhaled ENO may better protect postnatal lung development from oxidative stress than NO. OBJECTIVES: To compare inhaled NO and ENO on hyperoxia-impaired postnatal lung development. METHODS: We treated newborn rats beginning at birth to air or 95% O(2) +/- 0.2-20.0 ppm ENO for 8 days, or to 10 ppm NO for 8 days. Pups treated with the optimum ENO dose, 10 ppm, and pups treated with 10 ppm NO were recovered in room air for 6 more days. MEASUREMENTS AND MAIN RESULTS: ENO and NO partly prevented 95% O(2)-induced airway neutrophil influx in lavage, but ENO had a greater effect than did NO in prevention of lung myeloperoxidase accumulation, and in expression of cytokine-induced neutrophil chemoattractant-1. Treatment with 10 ppm ENO, but not NO, for 8 days followed by recovery in air for 6 days prevented 95% O(2)-induced impairments of body weight, lung compliance, and alveolar development. CONCLUSIONS: Inhaled ENO conferred protection superior to inhaled NO against hyperoxia-induced inflammation. ENO prevented hyperoxia impairments of lung compliance and postnatal alveolar development in newborn rats.

Full Text

Duke Authors

Cited Authors

  • Auten, RL; Mason, SN; Whorton, MH; Lampe, WR; Foster, WM; Goldberg, RN; Li, B; Stamler, JS; Auten, KM

Published Date

  • August 1, 2007

Published In

Volume / Issue

  • 176 / 3

Start / End Page

  • 291 - 299

PubMed ID

  • 17478622

Pubmed Central ID

  • 17478622

International Standard Serial Number (ISSN)

  • 1073-449X

Digital Object Identifier (DOI)

  • 10.1164/rccm.200605-662OC

Language

  • eng

Conference Location

  • United States