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Mycoplasma pneumoniae induces airway epithelial cell expression of MUC5AC in asthma.

Publication ,  Journal Article
Kraft, M; Adler, KB; Ingram, JL; Crews, AL; Atkinson, TP; Cairns, CB; Krause, DC; Chu, HW
Published in: Eur Respir J
January 2008

As excess mucin expression can contribute to the exacerbation of asthma, the present authors hypothesised that Mycoplasma pneumoniae significantly induces MUC5AC (the major airway mucin) expression in airway epithelial cells isolated directly from asthmatic subjects. A total of 11 subjects with asthma and six normal controls underwent bronchoscopy with airway brushing. Epithelial cells were cultured at an air-liquid interface and incubated with and without M. pneumoniae for 48 h, and in the presence and absence of nuclear factor (NF)-kappaB and a toll-like receptor (TLR)2 inhibitor. Quantitative PCR was performed for MUC5AC and TLR2 mRNA. MUC5AC protein and total protein were determined by ELISA. M. pneumoniae exposure significantly increased MUC5AC mRNA and protein expression after 48 h in epithelial cells isolated from asthmatic, but not from normal control subjects, at all concentrations as compared to unexposed cells. TLR2 mRNA expression was significantly increased in asthmatic epithelial cells at 4 h compared with unexposed cells. NF-kappaB and TLR2 inhibition reduced MUC5AC expression to the level of the unexposed control in both groups. Mycoplasma pneumoniae exposure significantly increased MUC5AC mRNA and protein expression preferentially in airway epithelial cells isolated from asthmatic subjects. The toll-like receptor 2 pathway may be involved in this process.

Duke Scholars

Published In

Eur Respir J

DOI

EISSN

1399-3003

Publication Date

January 2008

Volume

31

Issue

1

Start / End Page

43 / 46

Location

England

Related Subject Headings

  • Toll-Like Receptor 2
  • Time Factors
  • Respiratory System
  • NF-kappa B
  • Mycoplasma pneumoniae
  • Mucins
  • Mucin 5AC
  • Models, Biological
  • Male
  • Humans
 

Citation

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Chicago
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MLA
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Kraft, M., Adler, K. B., Ingram, J. L., Crews, A. L., Atkinson, T. P., Cairns, C. B., … Chu, H. W. (2008). Mycoplasma pneumoniae induces airway epithelial cell expression of MUC5AC in asthma. Eur Respir J, 31(1), 43–46. https://doi.org/10.1183/09031936.00103307
Kraft, M., K. B. Adler, J. L. Ingram, A. L. Crews, T. P. Atkinson, C. B. Cairns, D. C. Krause, and H. W. Chu. “Mycoplasma pneumoniae induces airway epithelial cell expression of MUC5AC in asthma.Eur Respir J 31, no. 1 (January 2008): 43–46. https://doi.org/10.1183/09031936.00103307.
Kraft M, Adler KB, Ingram JL, Crews AL, Atkinson TP, Cairns CB, et al. Mycoplasma pneumoniae induces airway epithelial cell expression of MUC5AC in asthma. Eur Respir J. 2008 Jan;31(1):43–6.
Kraft, M., et al. “Mycoplasma pneumoniae induces airway epithelial cell expression of MUC5AC in asthma.Eur Respir J, vol. 31, no. 1, Jan. 2008, pp. 43–46. Pubmed, doi:10.1183/09031936.00103307.
Kraft M, Adler KB, Ingram JL, Crews AL, Atkinson TP, Cairns CB, Krause DC, Chu HW. Mycoplasma pneumoniae induces airway epithelial cell expression of MUC5AC in asthma. Eur Respir J. 2008 Jan;31(1):43–46.
Journal cover image

Published In

Eur Respir J

DOI

EISSN

1399-3003

Publication Date

January 2008

Volume

31

Issue

1

Start / End Page

43 / 46

Location

England

Related Subject Headings

  • Toll-Like Receptor 2
  • Time Factors
  • Respiratory System
  • NF-kappa B
  • Mycoplasma pneumoniae
  • Mucins
  • Mucin 5AC
  • Models, Biological
  • Male
  • Humans