S-nitrosoglutathione reductase: an important regulator in human asthma.

Published

Journal Article

RATIONALE: Nitric oxide bioactivity, mediated through the formation of S-nitrosothiols (SNOs), has a significant effect on bronchomotor tone. S-Nitrosoglutathione is an endogenous bronchodilator that is decreased in children with asthmatic respiratory failure and in adults with asthma undergoing segmental airway challenge. Recently we showed that S-nitrosoglutathione reductase (GSNOR) regulates endogenous SNOs. Mice with genetic deletion of GSNOR are protected from airway hyperresponsivity in an allergic asthma model. OBJECTIVES: We hypothesized that GSNOR is increased in human asthma and correlates with lung SNO content and airway reactivity. METHODS: We recruited 36 subjects with mild asthma with FEV(1) 88.5 +/- 2.3% predicted and 34 healthy control subjects with FEV(1) 100.7 +/- 2.5% predicted. Bronchoalveolar lavage (BAL) was performed in all subjects. Cell counts, differentials, GSNOR activity, and SNO levels were determined in BAL. MEASUREMENTS AND MAIN RESULTS: SNO content was decreased in asthmatic BAL compared with control BAL and correlated inversely with GSNOR expression in BAL cell lysates. Furthermore, GSNOR activity measured from BAL samples was significantly increased in subjects with asthma compared with control subjects and correlated inversely with the provocative concentration of methacholine causing a 20% decrease in FEV(1). CONCLUSIONS: These findings suggest that GSNOR is an important regulator of airway SNO content and airways hyperresponsiveness in human asthma.

Full Text

Duke Authors

Cited Authors

  • Que, LG; Yang, Z; Stamler, JS; Lugogo, NL; Kraft, M

Published Date

  • August 1, 2009

Published In

Volume / Issue

  • 180 / 3

Start / End Page

  • 226 - 231

PubMed ID

  • 19395503

Pubmed Central ID

  • 19395503

Electronic International Standard Serial Number (EISSN)

  • 1535-4970

Digital Object Identifier (DOI)

  • 10.1164/rccm.200901-0158OC

Language

  • eng

Conference Location

  • United States