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Coordination of replication and transcription along a Drosophila chromosome.

Publication ,  Journal Article
MacAlpine, DM; Rodríguez, HK; Bell, SP
Published in: Genes Dev
December 15, 2004

The mechanisms by which metazoan origins of DNA replication are defined, regulated, and influenced by chromosomal events remain poorly understood. To gain insights into these mechanisms, we developed a systematic approach using a Drosophila high-resolution genomic microarray to determine replication timing, identify replication origins, and map protein-binding sites along a chromosome arm. We identify a defined temporal pattern of replication that correlates with the density of active transcription. These data indicate that the influence of transcription status on replication timing is exerted over large domains (>100 kb) rather than at the level of individual genes. We identify 62 early activating replication origins across the chromosome by mapping sites of nucleotide incorporation during hydroxyurea arrest. Using genome-wide location analysis, we demonstrate that the origin recognition complex (ORC) is localized to specific chromosomal sites, many of which coincide with early activating origins. The molecular attributes of ORC-binding sites include increased AT-content and association with a subset of RNA Pol II-binding sites. Based on these findings, we suggest that the distribution of transcription along the chromosome acts locally to influence origin selection and globally to regulate origin activation.

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Published In

Genes Dev

DOI

ISSN

0890-9369

Publication Date

December 15, 2004

Volume

18

Issue

24

Start / End Page

3094 / 3105

Location

United States

Related Subject Headings

  • Transcription, Genetic
  • Time Factors
  • Reverse Transcriptase Polymerase Chain Reaction
  • Replication Origin
  • RNA Polymerase II
  • Protein Binding
  • Origin Recognition Complex
  • Microarray Analysis
  • Immunoprecipitation
  • Fluorescence
 

Citation

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MacAlpine, D. M., Rodríguez, H. K., & Bell, S. P. (2004). Coordination of replication and transcription along a Drosophila chromosome. Genes Dev, 18(24), 3094–3105. https://doi.org/10.1101/gad.1246404
MacAlpine, David M., Heather K. Rodríguez, and Stephen P. Bell. “Coordination of replication and transcription along a Drosophila chromosome.Genes Dev 18, no. 24 (December 15, 2004): 3094–3105. https://doi.org/10.1101/gad.1246404.
MacAlpine DM, Rodríguez HK, Bell SP. Coordination of replication and transcription along a Drosophila chromosome. Genes Dev. 2004 Dec 15;18(24):3094–105.
MacAlpine, David M., et al. “Coordination of replication and transcription along a Drosophila chromosome.Genes Dev, vol. 18, no. 24, Dec. 2004, pp. 3094–105. Pubmed, doi:10.1101/gad.1246404.
MacAlpine DM, Rodríguez HK, Bell SP. Coordination of replication and transcription along a Drosophila chromosome. Genes Dev. 2004 Dec 15;18(24):3094–3105.

Published In

Genes Dev

DOI

ISSN

0890-9369

Publication Date

December 15, 2004

Volume

18

Issue

24

Start / End Page

3094 / 3105

Location

United States

Related Subject Headings

  • Transcription, Genetic
  • Time Factors
  • Reverse Transcriptase Polymerase Chain Reaction
  • Replication Origin
  • RNA Polymerase II
  • Protein Binding
  • Origin Recognition Complex
  • Microarray Analysis
  • Immunoprecipitation
  • Fluorescence