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Replication and preferential inheritance of hypersuppressive petite mitochondrial DNA.

Publication ,  Journal Article
MacAlpine, DM; Kolesar, J; Okamoto, K; Butow, RA; Perlman, PS
Published in: EMBO J
April 2, 2001

Wild-type yeast mitochondrial DNA (mtDNA) is inherited biparentally, whereas mtDNA of hypersuppressive petite mutants is inherited uniparentally in crosses to strains with wild-type mtDNA. Genomes of hypersuppressive petites contain a conserved ori sequence that includes a promoter, but it is unclear whether the ori confers a segregation or replication advantage. Fluorescent in situ hybridization analysis of wild-type and petite mtDNAs in crosses reveals no preferential segregation of hypersuppressive petite mtDNA to first zygotic buds. We identify single-stranded DNA circles and RNA-primed DNA replication intermediates in hypersuppressive petite mtDNA that are absent from non-hypersuppressive petites. Mutating the promoter blocks hypersuppressiveness in crosses to wild-type strains and eliminates the distinctive replication intermediates. We propose that promoter-dependent RNA-primed replication accounts for the uniparental inheritance of hypersuppressive petite mtDNA.

Duke Scholars

Published In

EMBO J

DOI

ISSN

0261-4189

Publication Date

April 2, 2001

Volume

20

Issue

7

Start / End Page

1807 / 1817

Location

England

Related Subject Headings

  • Transcription, Genetic
  • Saccharomyces cerevisiae
  • Replication Origin
  • Promoter Regions, Genetic
  • In Situ Hybridization, Fluorescence
  • Developmental Biology
  • DNA, Single-Stranded
  • DNA, Mitochondrial
  • DNA, Fungal
  • DNA, Circular
 

Citation

APA
Chicago
ICMJE
MLA
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MacAlpine, D. M., Kolesar, J., Okamoto, K., Butow, R. A., & Perlman, P. S. (2001). Replication and preferential inheritance of hypersuppressive petite mitochondrial DNA. EMBO J, 20(7), 1807–1817. https://doi.org/10.1093/emboj/20.7.1807
MacAlpine, D. M., J. Kolesar, K. Okamoto, R. A. Butow, and P. S. Perlman. “Replication and preferential inheritance of hypersuppressive petite mitochondrial DNA.EMBO J 20, no. 7 (April 2, 2001): 1807–17. https://doi.org/10.1093/emboj/20.7.1807.
MacAlpine DM, Kolesar J, Okamoto K, Butow RA, Perlman PS. Replication and preferential inheritance of hypersuppressive petite mitochondrial DNA. EMBO J. 2001 Apr 2;20(7):1807–17.
MacAlpine, D. M., et al. “Replication and preferential inheritance of hypersuppressive petite mitochondrial DNA.EMBO J, vol. 20, no. 7, Apr. 2001, pp. 1807–17. Pubmed, doi:10.1093/emboj/20.7.1807.
MacAlpine DM, Kolesar J, Okamoto K, Butow RA, Perlman PS. Replication and preferential inheritance of hypersuppressive petite mitochondrial DNA. EMBO J. 2001 Apr 2;20(7):1807–1817.

Published In

EMBO J

DOI

ISSN

0261-4189

Publication Date

April 2, 2001

Volume

20

Issue

7

Start / End Page

1807 / 1817

Location

England

Related Subject Headings

  • Transcription, Genetic
  • Saccharomyces cerevisiae
  • Replication Origin
  • Promoter Regions, Genetic
  • In Situ Hybridization, Fluorescence
  • Developmental Biology
  • DNA, Single-Stranded
  • DNA, Mitochondrial
  • DNA, Fungal
  • DNA, Circular