Replication and preferential inheritance of hypersuppressive petite mitochondrial DNA.

Published

Journal Article

Wild-type yeast mitochondrial DNA (mtDNA) is inherited biparentally, whereas mtDNA of hypersuppressive petite mutants is inherited uniparentally in crosses to strains with wild-type mtDNA. Genomes of hypersuppressive petites contain a conserved ori sequence that includes a promoter, but it is unclear whether the ori confers a segregation or replication advantage. Fluorescent in situ hybridization analysis of wild-type and petite mtDNAs in crosses reveals no preferential segregation of hypersuppressive petite mtDNA to first zygotic buds. We identify single-stranded DNA circles and RNA-primed DNA replication intermediates in hypersuppressive petite mtDNA that are absent from non-hypersuppressive petites. Mutating the promoter blocks hypersuppressiveness in crosses to wild-type strains and eliminates the distinctive replication intermediates. We propose that promoter-dependent RNA-primed replication accounts for the uniparental inheritance of hypersuppressive petite mtDNA.

Full Text

Duke Authors

Cited Authors

  • MacAlpine, DM; Kolesar, J; Okamoto, K; Butow, RA; Perlman, PS

Published Date

  • April 2, 2001

Published In

Volume / Issue

  • 20 / 7

Start / End Page

  • 1807 - 1817

PubMed ID

  • 11285243

Pubmed Central ID

  • 11285243

International Standard Serial Number (ISSN)

  • 0261-4189

Digital Object Identifier (DOI)

  • 10.1093/emboj/20.7.1807

Language

  • eng

Conference Location

  • England