Skip to main content

The histone modification pattern of active genes revealed through genome-wide chromatin analysis of a higher eukaryote.

Publication ,  Journal Article
Schübeler, D; MacAlpine, DM; Scalzo, D; Wirbelauer, C; Kooperberg, C; van Leeuwen, F; Gottschling, DE; O'Neill, LP; Turner, BM; Delrow, J ...
Published in: Genes Dev
June 1, 2004

The covalent modification of nucleosomal histones has emerged as a major determinant of chromatin structure and gene activity. To understand the interplay between various histone modifications, including acetylation and methylation, we performed a genome-wide chromatin structure analysis in a higher eukaryote. We found a binary pattern of histone modifications among euchromatic genes, with active genes being hyperacetylated for H3 and H4 and hypermethylated at Lys 4 and Lys 79 of H3, and inactive genes being hypomethylated and deacetylated at the same residues. Furthermore, the degree of modification correlates with the level of transcription, and modifications are largely restricted to transcribed regions, suggesting that their regulation is tightly linked to polymerase activity.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Genes Dev

DOI

ISSN

0890-9369

Publication Date

June 1, 2004

Volume

18

Issue

11

Start / End Page

1263 / 1271

Location

United States

Related Subject Headings

  • Transcription, Genetic
  • RNA
  • Oligonucleotide Array Sequence Analysis
  • Methylation
  • Histones
  • Genome
  • Gene Expression Profiling
  • Eukaryotic Cells
  • Drosophila melanogaster
  • Drosophila Proteins
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Schübeler, D., MacAlpine, D. M., Scalzo, D., Wirbelauer, C., Kooperberg, C., van Leeuwen, F., … Groudine, M. (2004). The histone modification pattern of active genes revealed through genome-wide chromatin analysis of a higher eukaryote. Genes Dev, 18(11), 1263–1271. https://doi.org/10.1101/gad.1198204
Schübeler, Dirk, David M. MacAlpine, David Scalzo, Christiane Wirbelauer, Charles Kooperberg, Fred van Leeuwen, Daniel E. Gottschling, et al. “The histone modification pattern of active genes revealed through genome-wide chromatin analysis of a higher eukaryote.Genes Dev 18, no. 11 (June 1, 2004): 1263–71. https://doi.org/10.1101/gad.1198204.
Schübeler D, MacAlpine DM, Scalzo D, Wirbelauer C, Kooperberg C, van Leeuwen F, et al. The histone modification pattern of active genes revealed through genome-wide chromatin analysis of a higher eukaryote. Genes Dev. 2004 Jun 1;18(11):1263–71.
Schübeler, Dirk, et al. “The histone modification pattern of active genes revealed through genome-wide chromatin analysis of a higher eukaryote.Genes Dev, vol. 18, no. 11, June 2004, pp. 1263–71. Pubmed, doi:10.1101/gad.1198204.
Schübeler D, MacAlpine DM, Scalzo D, Wirbelauer C, Kooperberg C, van Leeuwen F, Gottschling DE, O’Neill LP, Turner BM, Delrow J, Bell SP, Groudine M. The histone modification pattern of active genes revealed through genome-wide chromatin analysis of a higher eukaryote. Genes Dev. 2004 Jun 1;18(11):1263–1271.

Published In

Genes Dev

DOI

ISSN

0890-9369

Publication Date

June 1, 2004

Volume

18

Issue

11

Start / End Page

1263 / 1271

Location

United States

Related Subject Headings

  • Transcription, Genetic
  • RNA
  • Oligonucleotide Array Sequence Analysis
  • Methylation
  • Histones
  • Genome
  • Gene Expression Profiling
  • Eukaryotic Cells
  • Drosophila melanogaster
  • Drosophila Proteins