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Localized H3K36 methylation states define histone H4K16 acetylation during transcriptional elongation in Drosophila.

Publication ,  Journal Article
Bell, O; Wirbelauer, C; Hild, M; Scharf, AND; Schwaiger, M; MacAlpine, DM; Zilbermann, F; van Leeuwen, F; Bell, SP; Imhof, A; Garza, D ...
Published in: EMBO J
December 12, 2007

Post-translational modifications of histones are involved in transcript initiation and elongation. Methylation of lysine 36 of histone H3 (H3K36me) resides promoter distal at transcribed regions in Saccharomyces cerevisiae and is thought to prevent spurious initiation through recruitment of histone-deacetylase activity. Here, we report surprising complexity in distribution, regulation and readout of H3K36me in Drosophila involving two histone methyltransferases (HMTases). Dimethylation of H3K36 peaks adjacent to promoters and requires dMes-4, whereas trimethylation accumulates toward the 3' end of genes and relies on dHypb. Reduction of H3K36me3 is lethal in Drosophila larvae and leads to elevated levels of acetylation, specifically at lysine 16 of histone H4 (H4K16ac). In contrast, reduction of both di- and trimethylation decreases lysine 16 acetylation. Thus di- and trimethylation of H3K36 have opposite effects on H4K16 acetylation, which we propose enable dynamic changes in chromatin compaction during transcript elongation.

Duke Scholars

Published In

EMBO J

DOI

EISSN

1460-2075

Publication Date

December 12, 2007

Volume

26

Issue

24

Start / End Page

4974 / 4984

Location

England

Related Subject Headings

  • Transcription, Genetic
  • RNA Interference
  • Protein Processing, Post-Translational
  • Promoter Regions, Genetic
  • Oligonucleotide Array Sequence Analysis
  • Nuclear Proteins
  • Methylation
  • Lysine
  • Humans
  • Histones
 

Citation

APA
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Bell, O., Wirbelauer, C., Hild, M., Scharf, A. N. D., Schwaiger, M., MacAlpine, D. M., … Schübeler, D. (2007). Localized H3K36 methylation states define histone H4K16 acetylation during transcriptional elongation in Drosophila. EMBO J, 26(24), 4974–4984. https://doi.org/10.1038/sj.emboj.7601926
Bell, Oliver, Christiane Wirbelauer, Marc Hild, Annette N. D. Scharf, Michaela Schwaiger, David M. MacAlpine, Frédéric Zilbermann, et al. “Localized H3K36 methylation states define histone H4K16 acetylation during transcriptional elongation in Drosophila.EMBO J 26, no. 24 (December 12, 2007): 4974–84. https://doi.org/10.1038/sj.emboj.7601926.
Bell O, Wirbelauer C, Hild M, Scharf AND, Schwaiger M, MacAlpine DM, et al. Localized H3K36 methylation states define histone H4K16 acetylation during transcriptional elongation in Drosophila. EMBO J. 2007 Dec 12;26(24):4974–84.
Bell, Oliver, et al. “Localized H3K36 methylation states define histone H4K16 acetylation during transcriptional elongation in Drosophila.EMBO J, vol. 26, no. 24, Dec. 2007, pp. 4974–84. Pubmed, doi:10.1038/sj.emboj.7601926.
Bell O, Wirbelauer C, Hild M, Scharf AND, Schwaiger M, MacAlpine DM, Zilbermann F, van Leeuwen F, Bell SP, Imhof A, Garza D, Peters AHFM, Schübeler D. Localized H3K36 methylation states define histone H4K16 acetylation during transcriptional elongation in Drosophila. EMBO J. 2007 Dec 12;26(24):4974–4984.

Published In

EMBO J

DOI

EISSN

1460-2075

Publication Date

December 12, 2007

Volume

26

Issue

24

Start / End Page

4974 / 4984

Location

England

Related Subject Headings

  • Transcription, Genetic
  • RNA Interference
  • Protein Processing, Post-Translational
  • Promoter Regions, Genetic
  • Oligonucleotide Array Sequence Analysis
  • Nuclear Proteins
  • Methylation
  • Lysine
  • Humans
  • Histones