Conserved nucleosome positioning defines replication origins.

Journal Article (Journal Article)

The origin recognition complex (ORC) specifies replication origin location. The Saccharomyces cerevisiae ORC recognizes the ARS (autonomously replicating sequence) consensus sequence (ACS), but only a subset of potential genomic sites are bound, suggesting other chromosomal features influence ORC binding. Using high-throughput sequencing to map ORC binding and nucleosome positioning, we show that yeast origins are characterized by an asymmetric pattern of positioned nucleosomes flanking the ACS. The origin sequences are sufficient to maintain a nucleosome-free origin; however, ORC is required for the precise positioning of nucleosomes flanking the origin. These findings identify local nucleosomes as an important determinant for origin selection and function.

Full Text

Duke Authors

Cited Authors

  • Eaton, ML; Galani, K; Kang, S; Bell, SP; MacAlpine, DM

Published Date

  • April 15, 2010

Published In

Volume / Issue

  • 24 / 8

Start / End Page

  • 748 - 753

PubMed ID

  • 20351051

Pubmed Central ID

  • PMC2854390

Electronic International Standard Serial Number (EISSN)

  • 1549-5477

Digital Object Identifier (DOI)

  • 10.1101/gad.1913210


  • eng

Conference Location

  • United States