Conserved nucleosome positioning defines replication origins.
Journal Article (Journal Article)
The origin recognition complex (ORC) specifies replication origin location. The Saccharomyces cerevisiae ORC recognizes the ARS (autonomously replicating sequence) consensus sequence (ACS), but only a subset of potential genomic sites are bound, suggesting other chromosomal features influence ORC binding. Using high-throughput sequencing to map ORC binding and nucleosome positioning, we show that yeast origins are characterized by an asymmetric pattern of positioned nucleosomes flanking the ACS. The origin sequences are sufficient to maintain a nucleosome-free origin; however, ORC is required for the precise positioning of nucleosomes flanking the origin. These findings identify local nucleosomes as an important determinant for origin selection and function.
Full Text
Duke Authors
Cited Authors
- Eaton, ML; Galani, K; Kang, S; Bell, SP; MacAlpine, DM
Published Date
- April 15, 2010
Published In
Volume / Issue
- 24 / 8
Start / End Page
- 748 - 753
PubMed ID
- 20351051
Pubmed Central ID
- 20351051
Electronic International Standard Serial Number (EISSN)
- 1549-5477
Digital Object Identifier (DOI)
- 10.1101/gad.1913210
Language
- eng
Conference Location
- United States