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Drosophila ORC localizes to open chromatin and marks sites of cohesin complex loading.

Publication ,  Journal Article
MacAlpine, HK; Gordân, R; Powell, SK; Hartemink, AJ; MacAlpine, DM
Published in: Genome Res
February 2010

The origin recognition complex (ORC) is an essential DNA replication initiation factor conserved in all eukaryotes. In Saccharomyces cerevisiae, ORC binds to specific DNA elements; however, in higher eukaryotes, ORC exhibits little sequence specificity in vitro or in vivo. We investigated the genome-wide distribution of ORC in Drosophila and found that ORC localizes to specific chromosomal locations in the absence of any discernible simple motif. Although no clear sequence motif emerged, we were able to use machine learning approaches to accurately discriminate between ORC-associated sequences and ORC-free sequences based solely on primary sequence. The complex sequence features that define ORC binding sites are highly correlated with nucleosome positioning signals and likely represent a preferred nucleosomal landscape for ORC association. Open chromatin appears to be the underlying feature that is deterministic for ORC binding. ORC-associated sequences are enriched for the histone variant, H3.3, often at transcription start sites, and depleted for bulk nucleosomes. The density of ORC binding along the chromosome is reflected in the time at which a sequence replicates, with early replicating sequences having a high density of ORC binding. Finally, we found a high concordance between sites of ORC binding and cohesin loading, suggesting that, in addition to DNA replication, ORC may be required for the loading of cohesin on DNA in Drosophila.

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Published In

Genome Res

DOI

EISSN

1549-5469

Publication Date

February 2010

Volume

20

Issue

2

Start / End Page

201 / 211

Location

United States

Related Subject Headings

  • Sequence Analysis, DNA
  • Promoter Regions, Genetic
  • Origin Recognition Complex
  • Drosophila melanogaster
  • Drosophila Proteins
  • Cohesins
  • Chromosomal Proteins, Non-Histone
  • Chromatin
  • Cell Line
  • Cell Cycle Proteins
 

Citation

APA
Chicago
ICMJE
MLA
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MacAlpine, H. K., Gordân, R., Powell, S. K., Hartemink, A. J., & MacAlpine, D. M. (2010). Drosophila ORC localizes to open chromatin and marks sites of cohesin complex loading. Genome Res, 20(2), 201–211. https://doi.org/10.1101/gr.097873.109
MacAlpine, Heather K., Raluca Gordân, Sara K. Powell, Alexander J. Hartemink, and David M. MacAlpine. “Drosophila ORC localizes to open chromatin and marks sites of cohesin complex loading.Genome Res 20, no. 2 (February 2010): 201–11. https://doi.org/10.1101/gr.097873.109.
MacAlpine HK, Gordân R, Powell SK, Hartemink AJ, MacAlpine DM. Drosophila ORC localizes to open chromatin and marks sites of cohesin complex loading. Genome Res. 2010 Feb;20(2):201–11.
MacAlpine, Heather K., et al. “Drosophila ORC localizes to open chromatin and marks sites of cohesin complex loading.Genome Res, vol. 20, no. 2, Feb. 2010, pp. 201–11. Pubmed, doi:10.1101/gr.097873.109.
MacAlpine HK, Gordân R, Powell SK, Hartemink AJ, MacAlpine DM. Drosophila ORC localizes to open chromatin and marks sites of cohesin complex loading. Genome Res. 2010 Feb;20(2):201–211.

Published In

Genome Res

DOI

EISSN

1549-5469

Publication Date

February 2010

Volume

20

Issue

2

Start / End Page

201 / 211

Location

United States

Related Subject Headings

  • Sequence Analysis, DNA
  • Promoter Regions, Genetic
  • Origin Recognition Complex
  • Drosophila melanogaster
  • Drosophila Proteins
  • Cohesins
  • Chromosomal Proteins, Non-Histone
  • Chromatin
  • Cell Line
  • Cell Cycle Proteins