Microscopic diffusion tensor imaging of the mouse brain.
Journal Article (Journal Article)
Diffusion tensor imaging (DTI) data at 43 mum isotropic resolution has been acquired on the intact adult mouse brain in 28-h scan time by using a streamlined protocol, including specimen fixation and staining, image acquisition, reconstruction, post-processing, and distribution. An intermediate registration of each component image is required to achieve the desired microscopic resolution. Multiple parameters have been derived, including fractional anisotropy, axial and radial diffusivity, and a color-coded orientation map of the primary eigenvector. Each DTI dataset was mapped to a common reference space to facilitate future standardized analysis. Fiber tracking has also been demonstrated, providing 3D connection information. This protocol to acquire high-resolution DTI data in a robust and repeatable fashion will serve as a foundation to quantitatively study mouse brain integrity and white matter architecture, at what we believe to be the highest spatial resolution yet attained.
Full Text
Duke Authors
Cited Authors
- Jiang, Y; Johnson, GA
Published Date
- April 1, 2010
Published In
Volume / Issue
- 50 / 2
Start / End Page
- 465 - 471
PubMed ID
- 20034583
Pubmed Central ID
- PMC2826147
Electronic International Standard Serial Number (EISSN)
- 1095-9572
Digital Object Identifier (DOI)
- 10.1016/j.neuroimage.2009.12.057
Language
- eng
Conference Location
- United States