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Common endothelial progenitor cell assays identify discrete endothelial progenitor cell populations.

Publication ,  Journal Article
Povsic, TJ; Zavodni, KL; Vainorius, E; Kherani, JF; Goldschmidt-Clermont, PJ; Peterson, ED
Published in: Am Heart J
February 2009

BACKGROUND: Multiple measures of endothelial progenitor cells (EPCs) have been described, but there has been limited study of the comparability of these assays. We sought to determine the reproducibility of and correlation between alternative EPC assay methodologies. METHODS: We simultaneously assessed EPC numbers in 140 patients undergoing cardiac catheterization using the 2 most commonly used culture techniques: endothelial cell outgrowth and colony-forming unit (CFU). In the final 77 patients, EPCs were also identified on the basis of cell surface marker expression (CD133, CD34, and vascular endothelial growth factor receptor-2 [VEGFR-2]) and aldehyde dehydrogenase (ALDH) activity. RESULTS: Endothelial progenitor cell enumeration based on fluorescence activated cell sorting was more precise than culture assays. There was limited correlation between EPC numbers determined using the 2 common culture-based assays; however, endothelial CFUs correlated with VEGFR-2 and CD34/VEGFR-2-expressing cells. Endothelial progenitor cells defined by expression of CD133, CD34, CD133/CD34, and ALDH activity correlated with each other, but not with VEGFR-2(+) cells. CONCLUSIONS: Endothelial progenitor cells can be broadly classified into 2 classes: VEGFR-2-expressing cells, which give rise to endothelial CFUs, and CD133/CD34 or ALDH(br) cells. These observations underscore the need for better assay standardization and a more precise definition of EPCs in cell therapy research.

Duke Scholars

Published In

Am Heart J

DOI

EISSN

1097-6744

Publication Date

February 2009

Volume

157

Issue

2

Start / End Page

335 / 344

Location

United States

Related Subject Headings

  • Vascular Endothelial Growth Factor Receptor-2
  • Stem Cells
  • Reproducibility of Results
  • Peptides
  • Humans
  • Glycoproteins
  • Endothelial Cells
  • Cell Lineage
  • Cardiovascular System & Hematology
  • Antigens, CD34
 

Citation

APA
Chicago
ICMJE
MLA
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Povsic, T. J., Zavodni, K. L., Vainorius, E., Kherani, J. F., Goldschmidt-Clermont, P. J., & Peterson, E. D. (2009). Common endothelial progenitor cell assays identify discrete endothelial progenitor cell populations. Am Heart J, 157(2), 335–344. https://doi.org/10.1016/j.ahj.2008.10.010
Povsic, Thomas J., Katherine L. Zavodni, Enrikas Vainorius, Jennifer F. Kherani, Pascal J. Goldschmidt-Clermont, and Eric D. Peterson. “Common endothelial progenitor cell assays identify discrete endothelial progenitor cell populations.Am Heart J 157, no. 2 (February 2009): 335–44. https://doi.org/10.1016/j.ahj.2008.10.010.
Povsic TJ, Zavodni KL, Vainorius E, Kherani JF, Goldschmidt-Clermont PJ, Peterson ED. Common endothelial progenitor cell assays identify discrete endothelial progenitor cell populations. Am Heart J. 2009 Feb;157(2):335–44.
Povsic, Thomas J., et al. “Common endothelial progenitor cell assays identify discrete endothelial progenitor cell populations.Am Heart J, vol. 157, no. 2, Feb. 2009, pp. 335–44. Pubmed, doi:10.1016/j.ahj.2008.10.010.
Povsic TJ, Zavodni KL, Vainorius E, Kherani JF, Goldschmidt-Clermont PJ, Peterson ED. Common endothelial progenitor cell assays identify discrete endothelial progenitor cell populations. Am Heart J. 2009 Feb;157(2):335–344.
Journal cover image

Published In

Am Heart J

DOI

EISSN

1097-6744

Publication Date

February 2009

Volume

157

Issue

2

Start / End Page

335 / 344

Location

United States

Related Subject Headings

  • Vascular Endothelial Growth Factor Receptor-2
  • Stem Cells
  • Reproducibility of Results
  • Peptides
  • Humans
  • Glycoproteins
  • Endothelial Cells
  • Cell Lineage
  • Cardiovascular System & Hematology
  • Antigens, CD34