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Aldehyde dehydrogenase activity allows reliable EPC enumeration in stored peripheral blood samples.

Publication ,  Journal Article
Povsic, TJ; Adams, SD; Zavodni, KL; Kelly, F; Melton, LG; Rao, SV; Najjar, SS; Harrington, RA; Peterson, ED
Published in: J Thromb Thrombolysis
October 2009

BACKGROUND: Interest in the biology of endogenous progenitor cells (EPCs) continues to grow as evidence of their role in vascular repair mounts. EPC enumeration requires specialized laboratory techniques and is performed immediately after sample acquisition, limiting the clinical contexts in which EPC enumeration can be performed and the ability to increase sample sizes through multi-center participation. METHODS: We compared the numbers of EPCs enumerated in samples processed immediately after acquisition (n = 36) with EPCs enumerated in specimens stored for 24 hours or after cryopreservation of mononuclear cells (MNC) using two EPC identification strategies: cell surface marker expression (CD133/CD34) and aldehyde dehydrogenase activity (ALDH(br) cells). RESULTS: EPCs assessed in fresh samples correlated with EPCs enumerated after whole blood storage (r = 0.699 for CD133(+)CD34(+) cells, r = 0.880 for ALDH(br) cells, P < 0.005 and P < 0.0001, respectively) or mononuclear cryopreservation (r = 0.590 for CD133(+)CD34(+) cells, r = 0.894 for ALDH(br) cells, P < 0.0001 for each); however, correlation based on assessment of ALDH(br) cells was higher (P < 0.0003 for comparison of correlation coefficients). Initial results from a multi-site clinical trial suggest that EPC enumeration after mononuclear cell cryopreservation is feasible. CONCLUSION: EPC analysis based on ALDH activity is reproducible, even after extended whole blood storage or MNC cryopreservation.

Duke Scholars

Published In

J Thromb Thrombolysis

DOI

EISSN

1573-742X

Publication Date

October 2009

Volume

28

Issue

3

Start / End Page

259 / 265

Location

Netherlands

Related Subject Headings

  • Stem Cells
  • Middle Aged
  • Male
  • Humans
  • Female
  • Endothelial Cells
  • Cell Count
  • Cardiovascular System & Hematology
  • Blood Preservation
  • Blood Cells
 

Citation

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Povsic, T. J., Adams, S. D., Zavodni, K. L., Kelly, F., Melton, L. G., Rao, S. V., … Peterson, E. D. (2009). Aldehyde dehydrogenase activity allows reliable EPC enumeration in stored peripheral blood samples. J Thromb Thrombolysis, 28(3), 259–265. https://doi.org/10.1007/s11239-009-0306-6
Povsic, Thomas J., Stacie D. Adams, Katherine L. Zavodni, Francine Kelly, Laura G. Melton, Sunil V. Rao, Samer S. Najjar, Robert A. Harrington, and Eric D. Peterson. “Aldehyde dehydrogenase activity allows reliable EPC enumeration in stored peripheral blood samples.J Thromb Thrombolysis 28, no. 3 (October 2009): 259–65. https://doi.org/10.1007/s11239-009-0306-6.
Povsic TJ, Adams SD, Zavodni KL, Kelly F, Melton LG, Rao SV, et al. Aldehyde dehydrogenase activity allows reliable EPC enumeration in stored peripheral blood samples. J Thromb Thrombolysis. 2009 Oct;28(3):259–65.
Povsic, Thomas J., et al. “Aldehyde dehydrogenase activity allows reliable EPC enumeration in stored peripheral blood samples.J Thromb Thrombolysis, vol. 28, no. 3, Oct. 2009, pp. 259–65. Pubmed, doi:10.1007/s11239-009-0306-6.
Povsic TJ, Adams SD, Zavodni KL, Kelly F, Melton LG, Rao SV, Najjar SS, Harrington RA, Peterson ED. Aldehyde dehydrogenase activity allows reliable EPC enumeration in stored peripheral blood samples. J Thromb Thrombolysis. 2009 Oct;28(3):259–265.
Journal cover image

Published In

J Thromb Thrombolysis

DOI

EISSN

1573-742X

Publication Date

October 2009

Volume

28

Issue

3

Start / End Page

259 / 265

Location

Netherlands

Related Subject Headings

  • Stem Cells
  • Middle Aged
  • Male
  • Humans
  • Female
  • Endothelial Cells
  • Cell Count
  • Cardiovascular System & Hematology
  • Blood Preservation
  • Blood Cells