Acute histologic effects of extraesophageal reflux on vocal fold healing.


Journal Article

This study evaluates how extraesophageal reflux affects membranous vocal fold healing in a canine model. We created membranous vocal fold injuries in the animals and randomly assigned them to topical application of acid and pepsin at pH 2 or pH 6 or of normal saline solution every other day for 12 days. The experimental vocal folds were compared to uninjured, control vocal folds from animals painlessly sacrificed for other reasons. Hematoxylin and eosin, fibronectin, and procollagen I staining were performed for histologic analysis. The injured specimens had three times greater cellular infiltrate (p < or = .001, analysis of variance) and twice as much fibronectin and procollagen I (p < or = .001, analysis of variance) as did the specimens from the control animals. No significant differences or trends were identified for cellular infiltrate, fibronectin, or procollagen I within the injured groups (p > .05, Bonferroni t-test). Acute wound healing did not appear to be influenced by the presence of acid and pepsin at pH 2 or 6 as compared to saline solution.

Full Text

Duke Authors

Cited Authors

  • Cohen, SM; Huang, S; Garrett, CG; Courey, MS

Published Date

  • April 2005

Published In

Volume / Issue

  • 114 / 4

Start / End Page

  • 296 - 303

PubMed ID

  • 15895785

Pubmed Central ID

  • 15895785

International Standard Serial Number (ISSN)

  • 0003-4894

Digital Object Identifier (DOI)

  • 10.1177/000348940511400408


  • eng

Conference Location

  • United States