A simple sample preparation for enhancing the sensitivity of mass spectrometric oligosaccharide determinations through the use of an adsorptive hydrophobic resin.


Journal Article

A simple microadsorption technique is described to remove detergent additives from oligosaccharide samples before their mass spectrometric analysis. The described methodology has been validated with submicrogram quantities of contaminated glycoproteins. This procedure is applicable to investigating minute quantities of glycans in both the positive- and negative-ion mode of matrix-assisted laser desorption/ionization mass spectrometry.

Full Text

Cited Authors

  • Huang, Y; Mechref, Y; Tian, J; Gong, H; Lennarz, WJ; Novotny, MV

Published Date

  • January 2000

Published In

Volume / Issue

  • 14 / 14

Start / End Page

  • 1233 - 1237

PubMed ID

  • 10918373

Pubmed Central ID

  • 10918373

Electronic International Standard Serial Number (EISSN)

  • 1097-0231

International Standard Serial Number (ISSN)

  • 0951-4198

Digital Object Identifier (DOI)

  • 10.1002/1097-0231(20000730)14:14<1233::aid-rcm16>3.0.co;2-p


  • eng