Fabrication of plastic biochips


Journal Article

A versatile surface functionalization procedure based on rf magnetron sputtering of silica was performed on poly(methylmethacrylate), polycarbonate, polypropylene, and cyclic olefin copolymers (Topas 6015). The hybrid thermoplastic surfaces were characterized by x-ray photoelectron spectrometer analysis and contact angle measurements. The authors then used these hybrid materials to perform a sandwich assay targeting an HIV-1 antibody using fluorescent detection and biotinylated peptides immobilized using the bioaffinity of biotin-neutravidin. They found a limit of detection similar to arrays on glass surfaces and believed that this plastic biochip platform may be used for the development of disposable immunosensing and diagnostic applications. © 2010 American Vacuum Society.

Full Text

Duke Authors

Cited Authors

  • Saaem, I; Ma, KS; Alam, SM; Tian, J

Published Date

  • July 1, 2010

Published In

Volume / Issue

  • 28 / 4

Start / End Page

  • 963 - 968

International Standard Serial Number (ISSN)

  • 0734-2101

Digital Object Identifier (DOI)

  • 10.1116/1.3432480

Citation Source

  • Scopus