Fabrication of plastic biochips
Published
Journal Article
A versatile surface functionalization procedure based on rf magnetron sputtering of silica was performed on poly(methylmethacrylate), polycarbonate, polypropylene, and cyclic olefin copolymers (Topas 6015). The hybrid thermoplastic surfaces were characterized by x-ray photoelectron spectrometer analysis and contact angle measurements. The authors then used these hybrid materials to perform a sandwich assay targeting an HIV-1 antibody using fluorescent detection and biotinylated peptides immobilized using the bioaffinity of biotin-neutravidin. They found a limit of detection similar to arrays on glass surfaces and believed that this plastic biochip platform may be used for the development of disposable immunosensing and diagnostic applications. © 2010 American Vacuum Society.
Full Text
Duke Authors
Cited Authors
- Saaem, I; Ma, KS; Alam, SM; Tian, J
Published Date
- July 1, 2010
Published In
Volume / Issue
- 28 / 4
Start / End Page
- 963 - 968
International Standard Serial Number (ISSN)
- 0734-2101
Digital Object Identifier (DOI)
- 10.1116/1.3432480
Citation Source
- Scopus